Animal-Free SUMO Protease Protein, Saccharomyces cerevisiae (His)

Animal-Free SUMO Protease Protein, Saccharomyces cerevisiae (His) is the recombinant SUMO Protease, expressed by E. coli, with His labeled tag.

10 U/μl .
Unit definition: One unit of SUMO Protease cleaves ≥ 85% of 2 μg control substrate in 1 h at 30°C.

Others

E. coli

His

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N/A

≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 25 mM Tris-HCl, 0.1% Igepal, NP-40, 250 mM NaCl, 0.5 mM DTT, 50% (v/v) glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

< 0.2 EU/μg of protein by gel clotting method

N/A.

Stored at -20°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -20°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.