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  4. Arylsulfatase A/ARSA Protein, Human (489a.a, HEK293, C-His)

Arylsulfatase A/ARSA Protein, Human (489a.a, HEK293, C-His)

Cat. No.: HY-P7608A
COA Handling Instructions

Arylsulfatase A (ARSA) protein specializes in hydrolyzing cerebroside sulfate, demonstrating its key role in breaking down this particular substrate. Arylsulfatase A/ARSA Protein, Human (489a.a, HEK293, C-His) is the recombinant human-derived Arylsulfatase A/ARSA protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Arylsulfatase A/ARSA Protein, Human (489a.a, HEK293, C-His) is 489 a.a., with molecular weight of approximately 65 kDa.

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  • Biological Activity

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Description

Arylsulfatase A (ARSA) protein specializes in hydrolyzing cerebroside sulfate, demonstrating its key role in breaking down this particular substrate. Arylsulfatase A/ARSA Protein, Human (489a.a, HEK293, C-His) is the recombinant human-derived Arylsulfatase A/ARSA protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Arylsulfatase A/ARSA Protein, Human (489a.a, HEK293, C-His) is 489 a.a., with molecular weight of approximately 65 kDa.

Background

Arylsulfatase A (ARSA) protein is specialized in the hydrolysis of cerebroside sulfate. This enzymatic function involves the cleavage of cerebroside sulfate, showcasing ARSA's role in the breakdown of this specific substrate.

Biological Activity

Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS). The specific activity is 9.75×105 pmol/min/µg, as measured under the described conditions.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

P15289 (R19-A507)

Gene ID

410  [NCBI]

Molecular Construction
N-term
ARSA (R19-A507)
Accession # P15289
6*His
C-term
Synonyms
rHuARSA, His; Arylsulfatase A; ARSA
AA Sequence

RPPNIVLIFADDLGYGDLGCYGHPSSTTPNLDQLAAGGLRFTDFYVPVSLCTPSRAALLTGRLPVRMGMYPGVLVPSSRGGLPLEEVTVAEVLAARGYLTGMAGKWHLGVGPEGAFLPPHQGFHRFLGIPYSHDQGPCQNLTCFPPATPCDGGCDQGLVPIPLLANLSVEAQPPWLPGLEARYMAFAHDLMADAQRQDRPFFLYYASHHTHYPQFSGQSFAERSGRGPFGDSLMELDAAVGTLMTAIGDLGLLEETLVIFTADNGPETMRMSRGGCSGLLRCGKGTTYEGGVREPALAFWPGHIAPGVTHELASSLDLLPTLAALAGAPLPNVTLDGFDLSPLLLGTGKSPRQSLFFYPSYPDEVRGVFAVRTGKYKAHFFTQGSAHSDTTADPACHASSSLTAHEPPLLYDLSKDPGENYNLLGGVAGATPEVLQALKQLQLLKAQLDAAVTFGPSQVARGEDPALQICCHPGCTPRPACCHCPDPHAHHHHHH

Molecular Weight

approximately 65 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCL, 150 mM NaCl, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Arylsulfatase A/ARSA Protein, Human (489a.a, HEK293, C-His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Arylsulfatase A/ARSA Protein, Human (489a.a, HEK293, C-His)
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HY-P7608A
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