B7-1/CD80 Protein, Human (Biotinylated, HEK293, His-Avi)

The CD80 protein plays a key role in T lymphocyte activation, promoting proliferation and cytokine production through CD28 binding. In contrast, interaction with CTLA-4 inhibits T cell activation. B7-1/CD80 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived B7-1/CD80 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

The CD80 Protein plays a pivotal role in the costimulatory signal essential for T-lymphocyte activation, facilitating T-cell proliferation and cytokine production upon binding to CD28. Conversely, when interacting with CTLA-4, CD80 elicits opposite effects, inhibiting T-cell activation. In the context of microbial infection, CD80 also acts as a receptor for adenovirus subgroup B, further exemplifying its multifaceted involvement in immune responses and its recognition of diverse signaling cues. The intricate modulation of T-cell activation by CD80 underscores its significance in orchestrating adaptive immune responses and highlights its versatile functions in different physiological contexts.

1. Immobilized Biotinylated Human B7-1 His at 1 μg/mL (100 μL/Well) on the plate. Dose response curve for Human CTLA-4 hFc with the EC₅₀ 0.1 μg/mL determined by ELISA.
2.Immobilized Human CTLA-4,hFc Tag at 2 μg/mL (100 μL/Well) on the plate. Dose response curve for Biotinylated Human B7-1, His Tag with the EC₅₀ of 1.9 μg/mL determined by ELISA.

Human

HEK293

C-Avi;C-8*His

P33681-1 (V35-N242)

941  [NCBI]

Extracellular Domain

Biotin

VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDN

50-70 kDa

≥ 95%, as determined by Bis-Tris PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.