B7-1/CD80 Protein, Human (HEK293, His)

The CD80 protein plays a key role in T lymphocyte activation, promoting proliferation and cytokine production through CD28 binding. In contrast, interaction with CTLA-4 inhibits T cell activation. B7-1/CD80 Protein, Human (HEK293, His) is the recombinant human-derived B7-1/CD80 protein, expressed by HEK293 , with C-6*His labeled tag.

The CD80 Protein plays a pivotal role in the costimulatory signal essential for T-lymphocyte activation, facilitating T-cell proliferation and cytokine production upon binding to CD28. Conversely, when interacting with CTLA-4, CD80 elicits opposite effects, inhibiting T-cell activation. In the context of microbial infection, CD80 also acts as a receptor for adenovirus subgroup B, further exemplifying its multifaceted involvement in immune responses and its recognition of diverse signaling cues. The intricate modulation of T-cell activation by CD80 underscores its significance in orchestrating adaptive immune responses and highlights its versatile functions in different physiological contexts.

Measured by its binding ability in a functional ELISA. 1. Immobilized Human B7-1 at 0.5 μg/ml (100μl/well) can bind biotinylated Human CTLA-4 (HY-P72959). The ED₅₀ for this effect is 225.8 ng/mL.
2. When Human B7-1 is immobilized at 1 µg/mL (100 µL/well) can bind biotinylated Human CTLA-4 (HY-P70001). The ED₅₀ for this effect is 0.1846 μg/mL.

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  When Human B7-1 is immobilized at 1 µg/mL (100 µL/well) can bind biotinylated Human CTLA-4 (HY-P70001). The ED₅₀ for this effect is 0.1846 μg/mL.
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  CTLA-4 Protein, Human (HY-P70001) captured on CM5 Chip can bind B7-1/CD80 Protein, Human (HEK293, His) with an affinity constant of 1.86E-7 M as determined in SPR assay (Biacore 8K).

Human

HEK293

C-6*His

P33681-1 (V35-N242)

941  [NCBI]

Extracellular Domain

VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDN

38-60 kDa

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  ≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.