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B7-1/CD80 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

The CD80 protein plays a key role in T lymphocyte activation, promoting proliferation and cytokine production through CD28 binding. In contrast, interaction with CTLA-4 inhibits T cell activation. B7-1/CD80 Protein, Human (HEK293, His) is the recombinant human-derived B7-1/CD80 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for B7-1/CD80 Protein, Human (HEK293, His)
  • Experimental Validation Results for B7-1/CD80 Protein, Human (HEK293, His)
  • Experimental Validation Results for B7-1/CD80 Protein, Human (HEK293, His)
  • B7-1/CD80 Protein, Human (HEK293, His) Product Image
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Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Use of MCE Products

Biological Activity

Description

The CD80 protein plays a key role in T lymphocyte activation, promoting proliferation and cytokine production through CD28 binding. In contrast, interaction with CTLA-4 inhibits T cell activation. B7-1/CD80 Protein, Human (HEK293, His) is the recombinant human-derived B7-1/CD80 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

The CD80 Protein plays a pivotal role in the costimulatory signal essential for T-lymphocyte activation, facilitating T-cell proliferation and cytokine production upon binding to CD28. Conversely, when interacting with CTLA-4, CD80 elicits opposite effects, inhibiting T-cell activation. In the context of microbial infection, CD80 also acts as a receptor for adenovirus subgroup B, further exemplifying its multifaceted involvement in immune responses and its recognition of diverse signaling cues. The intricate modulation of T-cell activation by CD80 underscores its significance in orchestrating adaptive immune responses and highlights its versatile functions in different physiological contexts.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. 1. Immobilized Human B7-1 at 0.5 μg/ml (100μl/well) can bind biotinylated Human CTLA-4 (HY-P72959). The ED50 for this effect is 225.8 ng/mL.
2. When Human B7-1 is immobilized at 1 µg/mL (100 µL/well) can bind biotinylated Human CTLA-4 (HY-P70001). The ED50 for this effect is 0.1846 μg/mL.
3. Immobilized Biotinylated Human CTLA-4 (C-Fc-Avi) at 10 μg/mL (100 μL/well) can bind Recombinant Human B7-1 (C-6His).The ED50 of Recombinant Human B7-1 (C-6His) is 1.0-5.0 ug/mL.
4. Loaded Biotinylated Human CTLA-4 on Pro-A Biosensor, can bind Recombinant Human B7-1 with an affinity constant of 1.0-5.0 μM as determined in BLI assay.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    When Human B7-1 is immobilized at 1 µg/mL (100 µL/well) can bind biotinylated Human CTLA-4 (HY-P70001). The ED50 for this effect is 0.1846 μg/mL.

  • Bioactivity - SPR

    Bioactivity - SPR

    CTLA-4 Protein, Human (HY-P70001) captured on CM5 Chip can bind B7-1/CD80 Protein, Human (HEK293, His) with an affinity constant of 1.86E-7 M as determined in SPR assay (Biacore 8K).

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P33681-1 (V35-N242)

  • Gene ID
    941  [NCBI]
  • Molecular Construction
    • N-term
    • B7-1 (V35-N242)
      Accession # P33681-1
    • 6*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    CD80; B-Lymphocyte Activation Antigen B7; Prev. CD28LG1; LAB7; Prev. CD28LG; BB1; T-Lymphocyte Activation Antigen CD80; B7; Activation B7-1 Antigen; Costimulatory Molecule Variant IgV-CD80; B7.1; Costimulatory Factor CD80; B7-1; CD80 Molecule; CD80 Antige

  • AA Sequence

    VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDN

  • Predicted Molecular Mass

    24.7 kDa

  • Molecular Weight

    Approximately 38-60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     When Human B7-1 is immobilized at 1 µg/mL (100 µL/well) can bind biotinylated Human CTLA-4 (HY-P70001). The ED50 for this effect is 0.1846 μg/mL.

  • 1/16

    MedChemExpress Validation
     CTLA-4 Protein, Human (HY-P70001) captured on CM5 Chip can bind B7-1/CD80 Protein, Human (HEK293, His) with an affinity constant of 1.86E-7 M as determined in SPR assay (Biacore 8K).

  • B7-1/CD80 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for B7-1/CD80 Protein, Human (HEK293, His)
  • Experimental Validation Results for B7-1/CD80 Protein, Human (HEK293, His)
  • Experimental Validation Results for B7-1/CD80 Protein, Human (HEK293, His)
  • B7-1/CD80 Protein, Human (HEK293, His) Product Image