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  5. B7-1/CD80 Protein, Human (HEK293, Fc)

B7-1/CD80 Protein, Human (HEK293, Fc)

Cat. No.: HY-P7321
COA Handling Instructions

B7-1/CD80 Protein, Human (HEK293, Fc) is a polypeptide chain with the C-termimal human IgG1 Fc fragment produced in HEK293 cells. CD80 is a costimulatory molecule known for its role in T-cell activation and also in regulating normal and malignant B cellsactivity.

For research use only. We do not sell to patients.

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Description

B7-1/CD80 Protein, Human (HEK293, Fc) is a polypeptide chain with the C-termimal human IgG1 Fc fragment produced in HEK293 cells. CD80 is a costimulatory molecule known for its role in T-cell activation and also in regulating normal and malignant B cellsactivity.

Background

CD80 (B7-1) and CD86 (B7-2) are expressed as cell surface molecules by APCs and are responsible for delivering additional or second signals to T cells when they interact with their ligands CD28 and CD152 (CTLA-4). Expression of B7.1 (CD80) and B7.2 (CD86), two related moleculesbelonging to the Ig superfamily, appears crucial to the ability of the APCs to activate T cells[1].

Biological Activity

1.2 µg/mL (100 µL/well) of immoblized recombinant human CTLA-4-hFc or CD28, hFc, Human can bind biotinylated human B7-1/CD80-Fc with a linear range of 1.22-9.77 ng/mL.
2.Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.09708 μg/mL in the presence of PHA, corresponding to a specific activity is 10300.783 U/mg.

Species

Human

Source

HEK293

Tag

C-hFc

Accession

P33681-1 (V35-N242)

Gene ID

941  [NCBI]

Molecular Construction
N-term
B7-1 (V35-N242)
Accession # P33681
hFc
C-term
Synonyms
rHuB7-1/CD80, Fc Chimera; BB1; CD28LG; CD28LG1; LAB7
AA Sequence

VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDN

Molecular Weight

Approximately 73-85 kDa due to the glycosylation.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized after extensive dialysis against PBS.

Endotoxin Level

<0.2 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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B7-1/CD80 Protein, Human (HEK293, Fc)
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HY-P7321
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