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  4. CA12/Carbonic Anhydrase 12 Protein, Cynomolgus (HEK293, His)

CA12/Carbonic Anhydrase 12 Protein, Cynomolgus (HEK293, His)

Cat. No.: HY-P703776
Instruction de manipulation Technical Support

CA12/Carbonic anhydrase 12 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived CA12/Carbonic anhydrase XII protein, expressed by HEK293, with C-His tag.

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Description

CA12/Carbonic anhydrase 12 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived CA12/Carbonic anhydrase XII protein, expressed by HEK293, with C-His tag.
Background

CA12 (Carbonic anhydrase XII) is a carbonic anhydrase enzyme that primarily catalyzes the reversible hydration of carbon dioxide. It plays roles in various physiological processes including pH regulation and ion transport. by similar: Other carbonic anhydrase family members (e.g., CA2 and CA9) are also involved in similar biochemical reactions.
Species

Cynomolgus
Source

HEK293
Tag

C-8*His
Accession

A0A2K5TSB2 (A25-G299)
Protein Length

Extracellular Domain
AA Sequence

APANGSKWTYLGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLMPLEFQGYNLSANEQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGPFNPSYDKIFSHLQHVKYKGQEAFIPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQVQAYTAAG

Predicted Molecular Mass
32 kDa
Masse moléculaire

Approximately 40-50 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.
Pureté

≥ 95%, as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Instruction de manipulation (2659 KB)

CA12/Carbonic Anhydrase 12 Protein, Cynomolgus (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CA12/Carbonic Anhydrase 12 Protein, Cynomolgus (HEK293, His)
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