CA12/Carbonic Anhydrase 12 Protein, Human (P.pastoris, His)

CA12 (or carbonic anhydrase 12) plays a crucial role in the reversible hydration of carbon dioxide, catalyzing its conversion into bicarbonate ions and protons. This enzymatic activity is essential for key physiological processes, including regulating pH and maintaining acid-base balance. CA12/Carbonic Anhydrase 12 Protein, Human (P.pastoris, His) is the recombinant human-derived CA12/Carbonic Anhydrase 12 protein, expressed by P. pastoris , with N-6*His labeled tag.

The CA12 protein, also known as Carbonic Anhydrase 12, plays a pivotal role in the reversible hydration of carbon dioxide. This enzyme catalyzes the conversion of carbon dioxide to bicarbonate ions and protons, contributing significantly to essential physiological processes. Its enzymatic activity is integral to the regulation of pH levels, aiding in the maintenance of acid-base balance within the body. As a member of the carbonic anhydrase family, CA12 is involved in the fundamental biochemical reactions related to carbon dioxide transport and buffering in tissues, underscoring its importance in cellular homeostasis.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

P. pastoris

N-6*His

O43570 (A25-S301)

771  [NCBI]

Extracellular Domain

APVNGSKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQVQVCTAAGLS

Approximately 33.1 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HC1, 0.5 M NaCl, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.