CA12/Carbonic Anhydrase 12 Protein, Human (HEK293, His)

CA12 (or carbonic anhydrase 12) plays a crucial role in the reversible hydration of carbon dioxide, catalyzing its conversion into bicarbonate ions and protons. This enzymatic activity is essential for key physiological processes, including regulating pH and maintaining acid-base balance. CA12/Carbonic Anhydrase 12 Protein, Human (HEK293, His) is the recombinant human-derived CA12/Carbonic Anhydrase 12 protein, expressed by HEK293 , with C-His labeled tag.

The CA12 protein, also known as Carbonic Anhydrase 12, plays a pivotal role in the reversible hydration of carbon dioxide. This enzyme catalyzes the conversion of carbon dioxide to bicarbonate ions and protons, contributing significantly to essential physiological processes. Its enzymatic activity is integral to the regulation of pH levels, aiding in the maintenance of acid-base balance within the body. As a member of the carbonic anhydrase family, CA12 is involved in the fundamental biochemical reactions related to carbon dioxide transport and buffering in tissues, underscoring its importance in cellular homeostasis.

Measured by its esterase activity and the specific activity is >40 pmoles/min/μg.

Human

HEK293

C-His

O43570 (A25-Q291)

771  [NCBI]

Partial

MPRRSLHAAAVLLLVILKEQPSSPAPVNGSKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQ

40-45 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.