1. Recombinant Proteins
  2. CAR-T Related Proteins Enzymes & Regulators
  3. Lyases (EC 4) Carbonic Anhydrase
  4. Carbonic Anhydrase 9 (CA IX)
  5. Carbonic Anhydrase 9 Protein, Cynomolgus (HEK293, His)

Carbonic Anhydrase 9 Protein, Cynomolgus (HEK293, His)

Cat. No.: HY-P700882
COA Handling Instructions

Carbonic Anhydrase 9 Protein catalyzes the reversible hydration of carbon dioxide, essential for converting it to bicarbonate ions and protons, and vice versa. This enzymatic activity is crucial in physiological processes like acid-base balance regulation, respiration, and cellular pH homeostasis. Carbonic Anhydrase 9 Protein is vital for carbon dioxide transport and metabolism, contributing to overall regulation of its levels in the body. Carbonic Anhydrase 9 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Carbonic Anhydrase 9 protein, expressed by HEK293 , with C-His labeled tag. The total length of Carbonic Anhydrase 9 Protein, Cynomolgus (HEK293, His) is 365 a.a., with molecular weight of 50-60 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

Carbonic Anhydrase 9 Protein catalyzes the reversible hydration of carbon dioxide, essential for converting it to bicarbonate ions and protons, and vice versa. This enzymatic activity is crucial in physiological processes like acid-base balance regulation, respiration, and cellular pH homeostasis. Carbonic Anhydrase 9 Protein is vital for carbon dioxide transport and metabolism, contributing to overall regulation of its levels in the body. Carbonic Anhydrase 9 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Carbonic Anhydrase 9 protein, expressed by HEK293 , with C-His labeled tag. The total length of Carbonic Anhydrase 9 Protein, Cynomolgus (HEK293, His) is 365 a.a., with molecular weight of 50-60 kDa.

Background

Carbonic Anhydrase 9 Protein is an enzyme that facilitates the reversible hydration of carbon dioxide. It plays a crucial role in converting carbon dioxide to bicarbonate ions and protons, as well as their reverse reaction. This enzymatic activity is essential for various physiological processes such as acid-base balance regulation, respiration, and maintenance of cellular pH homeostasis. Carbonic Anhydrase 9 Protein is vital in the transport and metabolism of carbon dioxide within the body, contributing to the overall regulation of carbon dioxide levels.

Biological Activity

Immobilized Cynomolgus CA9, His Tag at 0.5 μg/mL (100μl/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of <10.1 ng/mL determined by ELISA.

Species

Cynomolgus

Source

HEK293

Tag

C-His

Accession

A0A2K5VQG9 (Q38-D402)

Gene ID

/

Molecular Construction
N-term
CA9 (Q38-D402)
Accession # A0A2K5VQG9
His
C-term
Synonyms
CA9; CAIX; CA-IX; EC 4.2.1.1; G250; MN; P54/58N; PMW1; RCC
Molecular Weight

50-60 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Carbonic Anhydrase 9 Protein, Cynomolgus (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Carbonic Anhydrase 9 Protein, Cynomolgus (HEK293, His)
Cat. No.:
HY-P700882
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