Cardiac troponin I/Tnni3 Protein, Mouse (His-SUMO)

Cardiac troponin I, also known as Tnni3, plays a key role as an inhibitory subunit in the troponin complex, a key regulator of calcium sensitivity in actomyosin ATPase activity in striated muscle.Tnni3 interacts with TRIM63, emphasizing its involvement in complex cellular processes.Cardiac troponin I/Tnni3 Protein, Mouse (His-SUMO) is the recombinant mouse-derived Cardiac troponin I/Tnni3 protein, expressed by E.coli , with N-SUMO, N-6*His labeled tag.

Cardiac troponin I, also known as Tnni3, plays a pivotal role as the inhibitory subunit within the troponin complex, a critical regulator of calcium sensitivity in the actomyosin ATPase activity of striated muscle. This protein interacts with TRIM63, highlighting its involvement in intricate cellular processes. Furthermore, it exhibits binding affinity to actin and tropomyosin, integral components in the regulation of muscle contraction. The interaction with STK4/MST1 further suggests a network of associations that contribute to the multifaceted functions of cardiac troponin I in orchestrating the intricate machinery governing muscle physiology.

Mouse

E. coli

N-SUMO;N-6*His

P48787 (A2-G211)

21954  [NCBI]

Full Length of Mature Protein

ADESSDAAGEPQPAPAPVRRRSSANYRAYATEPHAKKKSKISASRKLQLKTLMLQIAKQEMEREAEERRGEKGRVLRTRCQPLELDGLGFEELQDLCRQLHARVDKVDEERYDVEAKVTKNITEIADLTQKIYDLRGKFKRPTLRRVRISADAMMQALLGTRAKESLDLRAHLKQVKKEDIEKENREVGDWRKNIDALSGMEGRKKKFEG

Approximately 45 kDa, based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.