Cas9 Protein (NLS, EGFP)

Cas9 protein is part of an immune system that defends against genetic elements. It processes RNA and aids in cleaving DNA targets. Protein and guide RNAs are necessary for its function. The protein recognizes specific sequences to distinguish self from nonself and provides immunity against matching genetic elements. Cas9 Protein (NLS, EGFP) is the recombinant Cas9 protein, expressed by E. coli.

Cas9, a revolutionary molecular tool, constitutes an adaptive immune system that defends against mobile genetic elements like viruses, transposable elements, and conjugative plasmids. The process involves transcription and processing of crRNA, wherein, for type II gene editing systems, the proper processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc), and the pivotal Cas9 protein. The tracrRNA guides ribonuclease 3 in processing pre-crRNA, with Cas9 stabilizing the pre-crRNA:tracrRNA interaction. In subsequent steps, Cas9/crRNA/tracrRNA complex acts as an endonuclease, cleaving the target DNA with complementary spacer sequences. Cas9 is inactive without the guidance of two specific guide RNAs (gRNA), emphasizing the necessity of both gRNAs for DNA binding and nuclease activity. This sophisticated system confers immunity against invading genetic elements through a mechanism known as gene editing interference.

E. coli

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≥ 95%, as determined by reducing SDS-PAGE.

<0.1 EU/μg, determined by LAL method.

• [1]. /biology-dictionary/hela-cell.html  [Content Brief]