CD200R1 Protein, Human (Biotinylated, HEK293, Fc-Avi)

CD200R1 protein is an inhibitory receptor that regulates key interactions by binding to the CD200/OX2 glycoprotein, thereby limiting inflammation by inhibiting TNF-α, interferons, and iNOS. CD200R1 Protein, Human (Biotinylated, HEK293, Fc-Avi) is the recombinant human-derived CD200R1 protein, expressed by HEK293, with C-Avi, C-hFc labeled tag. The total length of CD200R1 Protein, Human (Biotinylated, HEK293, Fc-Avi) is 240 a.a., with molecular weight of 70-115 kDa.

CD200R1 is an inhibitory receptor that acts as a key regulator by binding to the CD200/OX2 cell surface glycoprotein. Its regulatory role extends to limiting inflammation in response to specific stimuli by inhibiting pro-inflammatory molecules including TNF-α, interferons, and inducible nitric oxide synthase (iNOS). Interestingly, CD200R1 exhibits comparable affinity and kinetics when binding to the human herpesvirus 8 K14 viral CD200 homolog, mirroring its interaction with host CD200. The interaction between CD200 and CD200R1 is facilitated by their respective N-terminal Ig-like domains. In addition, CD200R1 binds to the human herpesvirus 8 vOX2 protein, further extending its multifunctional interactions in cellular processes.

Immobilized Human CD200 Fc at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD200 R1 Fc-Avi with a linear range of 0.5-7.8 ng/mL.

Human

HEK293

C-Avi;C-hFc

AAQ19772 (A27-L266)

131450  [NCBI]

Biotin

AAQPNNSLMLQTSKENHALASSSLCMDEKQITQNYSKVLAEVNTSWPVKMATNAVLCCPPIALRNLIIITWEIILRGQPSCTKAYRKETNETKETNCTDERITWVSRPDQNSDLQIRTVAITHDGYYRCIMVTPDGNFHRGYHLQVLVTPEVTLFQNRNRTAVCKAVAGKPAAHISWIPEGDCATKQEYWSNGTVTVKSTCHWEVHNVSTVTCHVSHLTGNKSLYIELLPVPGAKKSAKL

70-115 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of Tris with Glycine, Arginine and NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.