CD26/Dipeptidyl Peptidase 4 Protein, Cynomolgus (HEK293, Fc)

CD26/Dipeptidyl Peptidase 4 Protein is a serine membrane anchored exopeptidase, belonging to dipeptidyl peptidase (DPP) family. CD26/Dipeptidyl Peptidase 4 Protein is widely expressed in a variety of tissues and many cells^[1]. CD26/Dipeptidyl Peptidase 4 Protein, Cynomolgus (HEK293, Fc) is the recombinant cynomolgus-derived CD26/Dipeptidyl Peptidase 4 protein, expressed by HEK293 , with N-hFc labeled tag. The total length of CD26/Dipeptidyl Peptidase 4 Protein, Cynomolgus (HEK293, Fc) is 733 a.a., with molecular weight of ~113 kDa.

Dipeptidyl peptidase 4 (DPP4), also known as T cell surface antigen CD26, is a ubiquitous enzyme in human. It is widely expressed in a variety of tissues (lung, brain, pancreas, kidney, blood vessels, prostate, uterus, thymus, lymph nodes and spleen) and many cells (epithelial cells, inner skin cells, immune cells), especially highly expressed in kidney and small intestine. It is a transmembrane glycoprotein composed of 766 amino acids with a relative molecular weight of 110kDa. Its basic structure mainly includes: intracellular N-terminal region, transmembrane region and extracellular region. The extracellular part consists of a flexible rod, a glycosylation rich region (binding region with anti-CD26 antibody, adenosine deaminase (ADA) and caveolin-1), a cysteine rich region (binding region with collagen and fibronectin) and a catalytic region composed of the catalytic triad Ser630, Asp708 and His740. DPP4 contains nine potential glycosylation sites for glycosylation modification. Among them, co-translational core N-glycosylation was significantly associated with DPP4 folding and stability, whereas N-terminal sialylation appeared to regulate more pathophysiological processes. Hypersialylated DDP4 is responsible for the development of HIV and rheumatoid arthritis, while undersialylated DPP4 is shown to be linked with lung cancer^[1].

Measured by its ability to cleave the fluorogenic peptide substrate Gly-Pro-AMC (GP-AMC) and the specific activity is > 3, 000 pmoles/min/μg.

Cynomolgus

HEK293

N-hFc

F6VRB0 (D34-P766)

654491  [NCBI]

Approximately 113 kDa

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 25  mM MES, 0.7 M NaCl, pH 4.9.. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.