1. Recombinant Proteins
  2. Cytokines and Growth Factors Immune Checkpoint Proteins CD Antigens
  3. TNF Superfamily Stimulatory Immune Checkpoint Molecules T Cell CD Proteins B Cell CD Proteins Macrophage CD Proteins Monocyte CD Proteins Dendritic Cell CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins
  4. TNF Receptor Superfamily CD40 GITR/CD357
  5. CD40 Protein, Mouse (HEK293, Fc)

CD40 Protein, Mouse (HEK293, Fc)

Cat. No.: HY-P72911
Handling Instructions

CD40 protein is a TNFSF5/CD40LG receptor that transduces signals through the TRAF6 and MAP3K8 pathways, activates ERK in macrophages and B cells, and induces immunoglobulin secretion. CD40 exists in monomeric and homodimeric forms, interacts with TRAF proteins (TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6) and crucially mediates cellular responses to external signals. CD40 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived CD40 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD40 Protein, Mouse (HEK293, Fc) is 174 a.a., with molecular weight of 50-58 kDa.

For research use only. We do not sell to patients.

Protein Expression Service

Size Stock
50 μg   Get quote  
100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

CD40 protein is a TNFSF5/CD40LG receptor that transduces signals through the TRAF6 and MAP3K8 pathways, activates ERK in macrophages and B cells, and induces immunoglobulin secretion. CD40 exists in monomeric and homodimeric forms, interacts with TRAF proteins (TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6) and crucially mediates cellular responses to external signals. CD40 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived CD40 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD40 Protein, Mouse (HEK293, Fc) is 174 a.a., with molecular weight of 50-58 kDa.

Background

CD40 Protein serves as the receptor for TNFSF5/CD40LG, transducing signals through TRAF6 and MAP3K8 pathways to activate ERK in macrophages and B cells, resulting in the induction of immunoglobulin secretion. Existing in both monomeric and homodimeric forms, CD40 Protein interacts with TRAF1, TRAF2, TRAF3, TRAF5, and TRAF6, playing a crucial role in mediating cellular responses to external signals. The interaction with TRAF6 and MAP3K8 is particularly essential for the activation of ERK and subsequent cellular processes.

Species

Mouse

Source

HEK293

Tag

C-hFc

Accession

P27512 (L20-R193)

Gene ID

21939  [NCBI]

Molecular Construction
N-term
CD40 (L20-R193)
Accession # P27512
hFc
C-term
Synonyms
Tumor Necrosis Factor Receptor Superfamily member 5; Bp50; CD40L Receptor; CDw40; TNFRSF5
AA Sequence

LGQCVTCSDKQYLHDGQCCDLCQPGSRLTSHCTALEKTQCHPCDSGEFSAQWNREIRCHQHRHCEPNQGLRVKKEGTAESDTVCTCKEGQHCTSKDCEACAQHTPCIPGFGVMEMATETTDTVCHPCPVGFFSNQSSLFEKCYPWTSCEDKNLEVLQKGTSQTNVICGLKSRMR

Molecular Weight

50-58 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
CD40 Protein, Mouse (HEK293, Fc)
Cat. No.:
HY-P72911
Quantity:
MCE Japan Authorized Agent: