1. Recombinant Proteins
  2. CD Antigens
  3. Epithelial cell CD Proteins
  4. CD52
  5. CD52 Protein, Cynomolgus (HEK293, Fc)

CD52 Protein, Cynomolgus (HEK293, Fc)

Cat. No.: HY-P76240
Handling Instructions

CD52 Protein carries and orients carbohydrates, indicating involvement in molecular transport and arrangement. It also has a specific role that is not fully understood. CD52's versatility prompts further investigation into its mechanisms and contributions. Understanding its targeted role can reveal insights into its functions and broader implications. CD52 Protein, Cynomolgus (HEK293, Fc) is the recombinant cynomolgus-derived CD52 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD52 Protein, Cynomolgus (HEK293, Fc) is 35 a.a., with molecular weight of 35-46 KDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

CD52 Protein carries and orients carbohydrates, indicating involvement in molecular transport and arrangement. It also has a specific role that is not fully understood. CD52's versatility prompts further investigation into its mechanisms and contributions. Understanding its targeted role can reveal insights into its functions and broader implications. CD52 Protein, Cynomolgus (HEK293, Fc) is the recombinant cynomolgus-derived CD52 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD52 Protein, Cynomolgus (HEK293, Fc) is 35 a.a., with molecular weight of 35-46 KDa.

Background

The CD52 protein exhibits a multifaceted role, encompassing the carrying and orienting of carbohydrates, which suggests involvement in molecular transport and arrangement. Beyond these general functions, CD52 also appears to fulfill a more specific role, the details of which are yet to be fully elucidated. The dual nature of CD52's activities underscores its versatility in cellular processes, prompting further investigation into the precise mechanisms and specific contributions it makes. Exploring the intricacies of CD52's more targeted role holds the potential to unveil novel insights, enhancing our comprehension of its intricate functions and broader implications in cellular and molecular contexts.

Biological Activity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus CD52 is immobilized at 1 µg/mL (100 µL/well) can bind Recombinant Human Siglec‑10 Protein. The ED50 for this effect is 3.562 μg/mL.

Species

Cynomolgus

Source

HEK293

Tag

C-hFc

Accession

F6QMS2 (Q20-S35)

Gene ID

714429  [NCBI]

Molecular Construction
N-term
CD52 (M1-S35)
Accession # F6QMS2
hFc
C-term
Synonyms
CAMPATH-1; CD52 antigen; CDw52; Epididymal secretory protein E5; He5
AA Sequence

QTGVTSQNATSQSSPS

Molecular Weight

Approximately 35-46 kDa due to the glycosylation.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CD52 Protein, Cynomolgus (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD52 Protein, Cynomolgus (HEK293, Fc)
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HY-P76240
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