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  5. CD83 Protein, Mouse (113a.a, HEK293, Fc)

CD83 Protein, Mouse (113a.a, HEK293, Fc)

Cat. No.: HY-P72704
COA Handling Instructions

CD83 protein may be a key player in antigen presentation and cellular interactions during lymphocyte activation, highlighting its importance in immune processes. It potentially orchestrates antigen presentation and acts as a monomer. Investigating CD83's mechanisms in antigen presentation and lymphocyte activation could provide insights into its crucial role in shaping immune responses and cellular interactions within the immune system. CD83 Protein, Mouse (113a.a, HEK293, Fc) is the recombinant mouse-derived CD83 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD83 Protein, Mouse (113a.a, HEK293, Fc) is 113 a.a., with molecular weight of 55-65 kDa.

For research use only. We do not sell to patients.

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Description

CD83 protein may be a key player in antigen presentation and cellular interactions during lymphocyte activation, highlighting its importance in immune processes. It potentially orchestrates antigen presentation and acts as a monomer. Investigating CD83's mechanisms in antigen presentation and lymphocyte activation could provide insights into its crucial role in shaping immune responses and cellular interactions within the immune system. CD83 Protein, Mouse (113a.a, HEK293, Fc) is the recombinant mouse-derived CD83 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD83 Protein, Mouse (113a.a, HEK293, Fc) is 113 a.a., with molecular weight of 55-65 kDa.

Background

The CD83 protein emerges as a potential key player in antigen presentation or the subsequent cellular interactions that occur following lymphocyte activation, underscoring its significance in immune processes. Its involvement suggests a potential role in orchestrating the presentation of antigens, a crucial step in immune recognition and response. Structurally, CD83 functions as a monomer, indicating its singular molecular form in executing its biological activities. Further exploration into the specific mechanisms by which CD83 participates in antigen presentation and lymphocyte activation could provide valuable insights into its pivotal role in shaping immune responses and cellular interactions within the immune system.

Species

Mouse

Source

HEK293

Tag

C-hFc

Accession

O88324 (M22-A134)

Gene ID

12522  [NCBI]

Molecular Construction
N-term
CD83 (M22-A134)
Accession # O88324
hFc
C-term
Synonyms
B-cell activation protein; CD83 antigen; hCD83; CD83; BL11
AA Sequence

MREVTVACSETADLPCTAPWDPQLSYAVSWAKVSESGTESVELPESKQNSSFEAPRRRAYSLTIQNTTICSSGTYRCALQELGGQRNLSGTVVLKVTGCPKEATESTFRKYRA

Molecular Weight

55-65 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CD83 Protein, Mouse (113a.a, HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD83 Protein, Mouse (113a.a, HEK293, Fc)
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HY-P72704
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