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Chemerin/RARRES2 Protein, Mouse (HEK293, His)

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Based on 1 publication(s) in Google Scholar

Chemerin/RARRES2 proteins bind to signaling receptors and are involved in various processes, including promotion of adipocyte differentiation, protein phosphorylation, and insulin receptor signaling. It plays a role in brown adipocyte differentiation and is located in the extracellular region. Chemerin/RARRES2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Chemerin/RARRES2 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for Chemerin/RARRES2 Protein, Mouse (HEK293, His)
  • Chemerin/RARRES2 Protein, Mouse (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

Chemerin/RARRES2 proteins bind to signaling receptors and are involved in various processes, including promotion of adipocyte differentiation, protein phosphorylation, and insulin receptor signaling. It plays a role in brown adipocyte differentiation and is located in the extracellular region. Chemerin/RARRES2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Chemerin/RARRES2 protein, expressed by HEK293 , with C-His labeled tag.

Background

The Chemerin/RARRES2 protein is predicted to possess signaling receptor binding activity and is involved in various processes, including positive regulation of fat cell differentiation, positive regulation of protein phosphorylation, and regulation of insulin receptor signaling pathway. It acts upstream of or within brown fat cell differentiation and is located in the extracellular region. Chemerin/RARRES2 is expressed in the genitourinary system and retina and shares orthology with human RARRES2 (retinoic acid receptor responder 2). Biased expression of Chemerin/RARRES2 is observed in adult liver (RPKM 313.8), placenta (RPKM 166.0), and 12 other tissues, according to the Alliance of Genome Resources in April 2022.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-His
  • Accession

    Q9DD06/NP_082128.1 (T17-S156)

  • Gene ID
    71660  [NCBI]
  • Molecular Construction
    • N-term
    • Chemerin (M1-S156)
      Accession # Q9DD06/NP_082128.1
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    RARRES2; HP10433; Retinoic Acid Receptor Responder 2; Retinoic Acid Receptor Responder (Tazarotene Induced) 2; Chemerin; Tazarotene-Induced Gene 2 Protein; TIG2; RAR-Responsive Protein TIG2; Retinoic Acid Receptor Responder Protein 2

  • AA Sequence

    FSLEASEEMEQEPCPAPISEQQEQVLTVALGQPVRLCCGRTERGRHWYKEGSRLASAGRVRGWRGRLEIASFLPEDAGRYLCLARGSMTVVHNLTLIMDDSLPSINNEDPKTLSSSSSGHSYLQQAPYWTHPQRMEKKLHAVPAGNTVKFRCPAAGNPMPTIHWLKNGQAFHGENRIGGIRLRHQHWSLVMESVVPSDRGTYTCLVENSLGSIRYSYLLDVLERSPHRPILQAGLPANTTAVVGSNVELLCKVYSDAQPHIQWLKHIVINGSSFGADGFPYVQVLKTTDINSSEVEVLYLRNVSAEDAGEYTCLAGNSIGLSYQSAWLTVLPAEEEDLAWTTATSEARYTD

  • Predicted Molecular Mass

    17.4 kDa

  • Molecular Weight

    Approximately 18-20 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • Chemerin/RARRES2 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Chemerin/RARRES2 Protein, Mouse (HEK293, His)
  • Chemerin/RARRES2 Protein, Mouse (HEK293, His) Product Image