Cutinase Protein, Thermobifida Fusca (His)

Cutinase protein catalyzes the hydrolysis of cutin, a plant cuticle polyester. Cutinase Protein, Thermobifida Fusca (His) is the recombinant Cutinase protein, expressed by E. coli , with C-6*His labeled tag.

The Cutinase protein assumes a pivotal role in cellular processes, catalyzing the hydrolysis of cutin, a key polyester comprising the plant cuticle structure. As documented in various studies, Cutinase exhibits esterase activity toward p-nitrophenol-linked aliphatic esters (pNP-aliphatic esters), displaying a preference for short-chain substrates. Moreover, Cutinase demonstrates versatility by hydrolyzing triglycerides like triacetin, tributyrin, tricaprin, and trilaurin, with a pronounced affinity for short-chain substrates. Additionally, this enzyme displays the ability to hydrolyze the hemicellulose xylan and exhibits proficiency in degrading the widely used plastic poly(ethylene terephthalate) (PET). Furthermore, Cutinase can depolymerize poly(epsilon-caprolactone) (PCL), a synthetic aliphatic biodegradable polyester, highlighting its role in various biodegradation processes. The broad substrate specificity of Cutinase underscores its significance in enzymatic reactions involved in natural polymer breakdown and synthetic polymer degradation, showcasing its potential applications in environmental and industrial contexts.

Measured by its ability to catalyze a substrate, p-nitrophenol succinate, which can be measured by absorbance at 410 nm. The specific activity is 42.67 U/mL, as measured under the described conditions.

Others

E. coli

C-6*His

E5BBQ3 (A1-F261, S58R, T176S)

/

Partial

ANPYERGPNPTDALLEARSGPFSVSEENVSRLSASGFGGGTIYYPRENNTYGAVAISPGYTGTEASIAWLGERIASHGFVVITIDTITTLDQPDSRAEQLNAALNHMINRASSTVRSRIDSSRLAVMGHSMGGGGSLRLASQRPDLKAAIPLTPWHLNKNWSSVTVPTLIIGADLDTIAPVATHAKPFYNSLPSSISKAYLELDGATHFAPNIPNKIIGKYSVAWLKRFVDNDTRYTQFLCPGPRDGLFGEVEEYRSTCPF

28-30 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Solution

1.Supplied as a 0.2 μm filtered solution of PBS, 50% glycerol, pH 7.4.
2.Supplied as a 0.2 μm filtered solution of 20 mM HAc-NaAc, 50% glycerol, 5% Mannitol, 0.02% Tween 80, pH 4.5.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. /biology-dictionary/mda-mb-435s-cell.html  [Content Brief]