DCK/Deoxycytidine kinase Protein, Human (His-T7)
Based on 1 Customer Validation
DCK/deoxycytidine kinase protein can effectively phosphorylate deoxyribonucleosides, including deoxycytidine, deoxyguanosine and deoxyadenosine, showing broad substrate specificity and no chiral selectivity. As an indispensable enzyme, DCK plays a crucial role in phosphorylating nucleoside analogues, which is critical in antiviral and chemotherapeutic treatments. DCK/Deoxycytidine kinase Protein, Human (His-T7) is the recombinant human-derived DCK/Deoxycytidine kinase protein, expressed by E. coli , with N-6*His, N-T7 labeled tag.
- Species: Human
- Source: E. coli
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
DCK/deoxycytidine kinase protein can effectively phosphorylate deoxyribonucleosides, including deoxycytidine, deoxyguanosine and deoxyadenosine, showing broad substrate specificity and no chiral selectivity. As an indispensable enzyme, DCK plays a crucial role in phosphorylating nucleoside analogues, which is critical in antiviral and chemotherapeutic treatments. DCK/Deoxycytidine kinase Protein, Human (His-T7) is the recombinant human-derived DCK/Deoxycytidine kinase protein, expressed by E. coli , with N-6*His, N-T7 labeled tag.
Background
DCK, known as deoxycytidine kinase, demonstrates its enzymatic prowess by effectively phosphorylating deoxyribonucleosides, including deoxycytidine, deoxyguanosine, and deoxyadenosine. With broad substrate specificity, this kinase does not exhibit selectivity based on the chirality of the substrate. Its significance extends to being an indispensable enzyme for the phosphorylation of various nucleoside analogs commonly utilized as antiviral and chemotherapeutic agents. The ability of DCK to catalyze the phosphorylation of nucleosides plays a crucial role in cellular processes and contributes to the therapeutic efficacy of nucleoside analog-based treatments.
Verified Bioactivity
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
-
Species Human
-
Source E. coli
-
Tag N-6*His;N-T7
-
Accession
P27707 (M1-L260)
-
Molecular Construction
-
N-term
-
6*His-T7
-
DCK (M1-L260)
Accession # P27707 -
C-term
-
-
Protein Length
Full Length
-
Synonyms
DCK; Deoxyadenosine Kinase; Deoxycytidine Kinase; Deoxyguanosine Kinase; Deoxynucleoside Kinase
-
AA Sequence
MATPPKRSCPSFSASSEGTRIKKISIEGNIAAGKSTFVNILKQLCEDWEVVPEPVARWCNVQSTQDEFEELTMSQKNGGNVLQMMYEKPERWSFTFQTYACLSRIRAQLASLNGKLKDAEKPVLFFERSVYSDRYIFASNLYESECMNETEWTIYQDWHDWMNNQFGQSLELDGIIYLQATPETCLHRIYLRGRNEEQGIPLEYLEKLHYKHESWLLHRTLKTNFDYLQEVPILTLDVNEDFKDKYESLVEKVKEFLSTL
-
Predicted Molecular Mass
34 kDa
-
Molecular Weight
Approximately 30 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of PBS, 50% Glycerol, 1 mM TCEP, pH 7.4.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
-
Data Sheet (236 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
