1. Recombinant Proteins
  2. Receptor Proteins
  3. Notch family
  4. Delta-like 1 (DLL1 )
  5. Delta-like protein 1/DLL1 Protein, Human (HEK293, His)

Delta-like protein 1/DLL1 Protein, Human (HEK293, His)

Cat. No.: HY-P7841
COA Handling Instructions

DLL1 protein is a transmembrane ligand for NOTCH1, NOTCH2 and NOTCH3 receptors and participates in cis- and trans-interactions. Trans interactions trigger mechanical forces involving clathrin-mediated endocytosis, ligand ubiquitination, EPN1 interactions, and actin polymerization. Delta-like protein 1/DLL1 Protein, Human (HEK293, His) is the recombinant human-derived Delta-like protein 1/DLL1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Delta-like protein 1/DLL1 Protein, Human (HEK293, His) is 523 a.a., with molecular weight of ~65.0 kDa.

For research use only. We do not sell to patients.

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Description

DLL1 protein is a transmembrane ligand for NOTCH1, NOTCH2 and NOTCH3 receptors and participates in cis- and trans-interactions. Trans interactions trigger mechanical forces involving clathrin-mediated endocytosis, ligand ubiquitination, EPN1 interactions, and actin polymerization. Delta-like protein 1/DLL1 Protein, Human (HEK293, His) is the recombinant human-derived Delta-like protein 1/DLL1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Delta-like protein 1/DLL1 Protein, Human (HEK293, His) is 523 a.a., with molecular weight of ~65.0 kDa.

Background

Delta-like protein 1 (DLL1) functions as a transmembrane ligand protein for NOTCH1, NOTCH2, and NOTCH3 receptors, engaging in both cis and trans interactions with the extracellular domain (ECD) of Notch receptors. Upon transinteraction, ligand cells exert mechanical force through clathrin-mediated endocytosis, involving ligand ubiquitination, EPN1 interaction, and actin polymerization. These events facilitate Notch receptor extracellular domain (NECD) transendocytosis, triggering Notch signaling, including cleavage, hyperphosphorylation, and nuclear accumulation of the intracellular domain of Notch receptors (NICD). DLL1 is vital for embryonic development, maintaining adult stem cells in various tissues and the immune system. Its involvement in intercellular communication regulates cell lineage, patterning, and morphogenesis, influencing differentiation and proliferation. In brain development, DLL1 plays a role in neuronal differentiation and neocortex development, while in cerebellar development, it regulates Bergmann glial monolayer formation. It also contributes to immune system development, muscle development, pancreatic cell development, arterial identity maintenance, angiogenesis, goblet cell differentiation, inner ear development, and nephron development through the Notch signaling pathway. DLL1 functions as a homodimer and interacts with various proteins, orchestrating a diverse array of cellular processes.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

O00548 (Q18-G540)

Gene ID
Synonyms
rHuDelta-like protein 1/DLL1, His; Delta-like protein 1; Drosophila Delta homolog 1; Delta1; H-Delta-1; DLL1
AA Sequence

QVWSSGVFELKLQEFVNKKGLLGNRNCCRGGAGPPPCACRTFFRVCLKHYQASVSPEPPCTYGSAVTPVLGVDSFSLPDGGGADSAFSNPIRFPFGFTWPGTFSLIIEALHTDSPDDLATENPERLISRLATQRHLTVGEEWSQDLHSSGRTDLKYSYRFVCDEHYYGEGCSVFCRPRDDAFGHFTCGERGEKVCNPGWKGPYCTEPICLPGCDEQHGFCDKPGECKCRVGWQGRYCDECIRYPGCLHGTCQQPWQCNCQEGWGGLFCNQDLNYCTHHKPCKNGATCTNTGQGSYTCSCRPGYTGATCELGIDECDPSPCKNGGSCTDLENSYSCTCPPGFYGKICELSAMTCADGPCFNGGRCSDSPDGGYSCRCPVGYSGFNCEKKIDYCSSSPCSNGAKCVDLGDAYLCRCQAGFSGRHCDDNVDDCASSPCANGGTCRDGVNDFSCTCPPGYTGRNCSAPVSRCEHAPCHNGATCHERGHRYVCECARGYGGPNCQFLLPELPPGPAVVDLTEKLEGQG

Molecular Weight

Approximately 65.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Hepes, 150 mM NaCl, 1 mM EDTA, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Delta-like protein 1/DLL1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Delta-like protein 1/DLL1 Protein, Human (HEK293, His)
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HY-P7841
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