Delta-like protein 1/DLL1 Protein, Human (HEK293, His)

DLL1 protein is a transmembrane ligand for NOTCH1, NOTCH2 and NOTCH3 receptors and participates in cis- and trans-interactions. Trans interactions trigger mechanical forces involving clathrin-mediated endocytosis, ligand ubiquitination, EPN1 interactions, and actin polymerization. Delta-like protein 1/DLL1 Protein, Human (HEK293, His) is the recombinant human-derived Delta-like protein 1/DLL1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Delta-like protein 1/DLL1 Protein, Human (HEK293, His) is 523 a.a., with molecular weight of ~65.0 kDa.

Delta-like protein 1 (DLL1) functions as a transmembrane ligand protein for NOTCH1, NOTCH2, and NOTCH3 receptors, engaging in both cis and trans interactions with the extracellular domain (ECD) of Notch receptors. Upon transinteraction, ligand cells exert mechanical force through clathrin-mediated endocytosis, involving ligand ubiquitination, EPN1 interaction, and actin polymerization. These events facilitate Notch receptor extracellular domain (NECD) transendocytosis, triggering Notch signaling, including cleavage, hyperphosphorylation, and nuclear accumulation of the intracellular domain of Notch receptors (NICD). DLL1 is vital for embryonic development, maintaining adult stem cells in various tissues and the immune system. Its involvement in intercellular communication regulates cell lineage, patterning, and morphogenesis, influencing differentiation and proliferation. In brain development, DLL1 plays a role in neuronal differentiation and neocortex development, while in cerebellar development, it regulates Bergmann glial monolayer formation. It also contributes to immune system development, muscle development, pancreatic cell development, arterial identity maintenance, angiogenesis, goblet cell differentiation, inner ear development, and nephron development through the Notch signaling pathway. DLL1 functions as a homodimer and interacts with various proteins, orchestrating a diverse array of cellular processes.

Human

HEK293

C-6*His

O00548 (Q18-G540)

28514  [NCBI]

QVWSSGVFELKLQEFVNKKGLLGNRNCCRGGAGPPPCACRTFFRVCLKHYQASVSPEPPCTYGSAVTPVLGVDSFSLPDGGGADSAFSNPIRFPFGFTWPGTFSLIIEALHTDSPDDLATENPERLISRLATQRHLTVGEEWSQDLHSSGRTDLKYSYRFVCDEHYYGEGCSVFCRPRDDAFGHFTCGERGEKVCNPGWKGPYCTEPICLPGCDEQHGFCDKPGECKCRVGWQGRYCDECIRYPGCLHGTCQQPWQCNCQEGWGGLFCNQDLNYCTHHKPCKNGATCTNTGQGSYTCSCRPGYTGATCELGIDECDPSPCKNGGSCTDLENSYSCTCPPGFYGKICELSAMTCADGPCFNGGRCSDSPDGGYSCRCPVGYSGFNCEKKIDYCSSSPCSNGAKCVDLGDAYLCRCQAGFSGRHCDDNVDDCASSPCANGGTCRDGVNDFSCTCPPGYTGRNCSAPVSRCEHAPCHNGATCHERGHRYVCECARGYGGPNCQFLLPELPPGPAVVDLTEKLEGQG

Approximately 65.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Hepes, 150 mM NaCl, 1 mM EDTA, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.