1. Recombinant Proteins
  2. Receptor Proteins Biotinylated Proteins
  3. Notch family
  4. Delta-like 4 (DLL4)
  5. Delta-like protein 4/DLL4 Protein, Human (Biotinylated, HEK293, His)

Delta-like protein 4/DLL4 Protein, Human (Biotinylated, HEK293, His)

Cat. No.: HY-P78116
Handling Instructions

For research use only. We do not sell to patients.

Protein Expression Service

Size Stock
100 μg Ask For Quote & Lead Time

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity
Immobilized Biotinylated Human DLL4 His at 1 μg/mL (100μL/Well) on the plate. Dose response curve for Anti-DLL4 Antibody hFc with the EC50 of 76.7 ng/mL determined by ELISA.
Species

Human

Source

HEK293

Tag

C-His

Accession

Q9NR61 (S27-P524)

Gene ID
Synonyms
DLL4; MGC126344; notch ligand DLL4; Delta4; Delta-like 4
Molecular Weight

62-68 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE. Greater than 95% as determined by HPLC.

Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of 20 mM PB,500 mM NaCl, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Delta-like protein 4/DLL4 Protein, Human (Biotinylated, HEK293, His) Related Classifications

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Delta-like protein 4/DLL4 Protein, Human (Biotinylated, HEK293, His)
Cat. No.:
HY-P78116
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