DERP1 Protein, Dermatophagoides pteronyssinus (C132A, N150E, HEK293, His)

DERP1 Protein, a thiol protease, selectively cleaves target proteins with distinct structural features, favoring substrates with a large hydrophobic side chain in the P2 position or basic residues. This specificity underscores DERP1's functional significance in cellular processes. DERP1 Protein, Dermatophagoides pteronyssinus (C132A, N150E, HEK293, His) is the recombinant DERP1 protein, expressed by HEK293 , with C-His labeled tag and C132A, N150E mutation.

DERP1 Protein, identified as a thiol protease, exhibits a distinct preference for substrates characterized by a large hydrophobic side chain in the P2 position or those containing basic residues. This specificity underscores its role in selectively cleaving target proteins with particular structural features, highlighting the enzyme's functional significance in cellular processes.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Others

HEK293

C-His

P08176 (M1-L320, C132A, N150E)

113791973  [NCBI]

Full Length

C132A, N150E

C132A: Loss of activity. N150E: Loss of N-glycosylation.

MKIVLAIASLLALSAVYARPSSIKTFEEYKKAFNKSYATFEDEEAARKNFLESVKYVQSNGGAINHLSDLSLDEFKNRFLMSAEAFEHLKTQFDLNAETNACSINGNAPAEIDLRQMRTVTPIRMQGGCGSAWAFSGVAATESAYLAYREQSLDLAEQELVDCASQHGCHGDTIPRGIEYIQHNGVVQESYYRYVAREQSCRRPNAQRFGISNYCQIYPPNVNKIREALAQTHSAIAVIIGIKDLDAFRHYDGRTIIQRDNGYQPNYHAVNIVGYSNAQGVDYWIVRNSWDTNWGDNGYGYFAANIDLMMIEEYPYVVIL

Approximately 35.7 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM NH₄HCO₃, pH 8.3.

<1 EU/μg, determined by LAL method.

N/A.

Shipping with dry ice.

• [1]. /biology-dictionary/ku812-cell-line-cell.html  [Content Brief]