DNase I Protein, Human (HEK293, His)

The DNase I protein is a serum endonuclease secreted by a variety of exocrine and endocrine organs and expressed in non-hematopoietic tissues. It preferentially selects protein-free DNA, plays a key role in apoptosis-induced cell death, and inhibits actin polymerization by specifically binding to G-actin. DNase I Protein, Human (HEK293, His) is the recombinant human-derived DNase I protein, expressed by HEK293 , with C-His labeled tag.

DNase I, a serum endonuclease, is secreted by a diverse array of exocrine and endocrine organs. It is expressed in non-hematopoietic tissues and exhibits a preference for cleaving protein-free DNA. Apart from its general role as an endonuclease, DNase I plays a crucial role in apoptosis-induced cell death. Additionally, it binds specifically to G-actin, hindering actin polymerization. In collaboration with DNASE1L3, DNase I is instrumental in the degradation of neutrophil extracellular traps (NETs), which primarily consist of DNA fibers and are released by neutrophils during inflammation. The degradation of intravascular NETs by DNase I and DNASE1L3 is essential to prevent the formation of clots that could obstruct blood vessels, thereby mitigating organ damage following inflammatory responses.

One unit is defined as the amount of DNase I that degrades DNA and causes an increase in absorbance at 260 nm of 0.001/minute and the specific activity is >5000 unit/mg.

Human

HEK293

C-6*His

P24855-1 (L23-K282)

1773  [NCBI]

Full Length

LKIAAFNIQTFGETKMSNATLVSYIVQILSRYDIALVQEVRDSHLTAVGKLLDNLNQDAPDTYHYVVSEPLGRNSYKERYLFVYRPDQVSAVDSYYYDDGCEPCGNDTFNREPAIVRFFSRFTEVREFAIVPLHAAPGDAVAEIDALYDVYLDVQEKWGLEDVMLMGDFNAGCSYVRPSQWSSIRLWTSPTFQWLIPDSADTTATPTHCAYDRIVVAGMLLRGAVVPDSALPFNFQAAYGLSDQLAQAISDHYPVEVMLK

Approximately 37 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/cho-k1-cell.html  [Content Brief]