Dxr/DXP reductoisomerase Protein, E.coli (Myc, His)

Dxr/DXP Reductoisomerase is pivotal in isoprenoid biosynthesis, catalyzing the rearrangement and reduction of DXP to yield MEP. This step is critical in forming isoprenoid precursors, essential for cellular processes and metabolite synthesis. The enzyme's activity emphasizes its significance in regulating isoprenoid biosynthesis and cellular functions. Dxr/DXP reductoisomerase Protein, E.coli (Myc, His) is the recombinant E. coli-derived Dxr/DXP reductoisomerase protein, expressed by E. coli , with C-Myc, N-10*His labeled tag.

Dxr/DXP reductoisomerase is a protein that plays a crucial role in the biosynthesis of isoprenoids. It catalyzes the NADPH-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP), a key intermediate in the non-mevalonate pathway, to yield 2-C-methyl-D-erythritol 4-phosphate (MEP). This enzymatic conversion represents a pivotal step in the formation of isoprenoid precursors, essential compounds involved in various cellular processes, including the synthesis of essential metabolites and signaling molecules. The activity of Dxr/DXP reductoisomerase underscores its significance in the regulation of isoprenoid biosynthesis and cellular function.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

E.coli

E. coli

C-Myc;N-10*His

P45568 (M1-S398)

945019  [NCBI]

Full Length

MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVEQCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIVGAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSLPQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKISVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDMRTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTALNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKEVMRLAS

Approximately 48.4 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.