Ebola virus VP40/Matrix VP40 Protein (YP_138522, His-MBP)

Ebola virus VP40 (matrix VP40 protein) centrally directs virus assembly and budding and has complex interactions with viral ribonucleocapsid and host ESCRT proteins (VPS4, PDCD6IP/ALIX, NEDD4, TGS101). Ebola virus VP40/Matrix VP40 Protein (YP_138522, His-MBP) is the recombinant Virus-derived Ebola virus VP40/Matrix VP40 protein, expressed by E. coli , with N-His, N-MBP labeled tag.

The Ebola virus VP40, also known as Matrix VP40 protein, plays a central role in virus particle assembly and budding, orchestrating intricate interactions with both viral and host components. It functions by interacting with the viral ribonucleocapsid and members of the host ESCRT system, including VPS4, PDCD6IP/ALIX, NEDD4, or TGS101, essential for efficient budding. Additionally, its association with the host E3 ubiquitin ligase SMURF2 facilitates virus budding. Notably, VP40 may contribute to immune cell dysfunction by being packaged into exosomes, diminishing the viability of recipient cells through RNAi suppression and exosome-bystander apoptosis. Existing in various oligomeric forms, such as homodimers, homohexamers critical for budding, and homooctamers involved in genome replication and RNA binding, VP40 undergoes dynamic structural transitions upon reorganization at the plasma membrane into a hexameric form using phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2). These hexamers are crucial for the budding process, while octamers play a role in genome replication and RNA binding. VP40 further interacts with host factors including TSG101, NEDD4, PDCD6IP/ALIX, SMURF2, ITCH, and nucleoprotein/NP, highlighting its pivotal role in governing virus assembly and egress.

Virus

E. coli

N-His;N-MBP

YP_138522 (M1-K326)

3160775  [NCBI]

Full Length

MRRVTVPTAPPAYADIGYPMSMLPIKSSRAVSGIQQKQEVLPGMDTPSNSMRPVADDNIDHTSHTPNGVASAFILEATVNVISGPKVLMKQIPIWLPLGIADQKTYSFDSTTAAIMLASYTITHFGKANNPLVRVNRLGQGIPDHPLRLLRMGNQAFLQEFVLPPVQLPQYFTFDLTALKLVTQPLPAATWTDETPSNLSGALRPGLSFHPKLRPVLLPGKTGKKGHVSDLTAPDKIQTIVNLMQDFKIVPIDPAKSIIGIEVPELLVHKLTGKKMSQKNGQPIIPVLLPKYIGLDPISPGDLTMVITPDYDDCHSPASCSYLSEK

≥ 95%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.

• [1]. /biology-dictionary/l-363-cell.html