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  3. Hydrolases (EC 3)
  4. ELANE Protein, Human (GST)

ELANE Protein, Human (GST)

Cat. No.: HY-P72179
COA Handling Instructions

ELANE is a serine protease that critically regulates natural killer cells, monocytes, and granulocytes by inhibiting C5a-dependent neutrophil enzyme release and chemotaxis, thereby shaping immune responses. It also inhibits pyroptosis by cleaving GSDMB, affecting the regulation of programmed cell death. ELANE Protein, Human (GST) is the recombinant human-derived ELANE protein, expressed by E. coli , with N-GST labeled tag. The total length of ELANE Protein, Human (GST) is 238 a.a., with molecular weight of ~52.6 kDa.

For research use only. We do not sell to patients.

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Description

ELANE is a serine protease that critically regulates natural killer cells, monocytes, and granulocytes by inhibiting C5a-dependent neutrophil enzyme release and chemotaxis, thereby shaping immune responses. It also inhibits pyroptosis by cleaving GSDMB, affecting the regulation of programmed cell death. ELANE Protein, Human (GST) is the recombinant human-derived ELANE protein, expressed by E. coli , with N-GST labeled tag. The total length of ELANE Protein, Human (GST) is 238 a.a., with molecular weight of ~52.6 kDa.

Background

ELANE, a serine protease, plays a crucial role in modulating the functions of natural killer cells, monocytes, and granulocytes. It acts by inhibiting C5a-dependent neutrophil enzyme release and chemotaxis, highlighting its regulatory role in immune responses. Additionally, ELANE participates in the inhibition of pyroptosis by promoting the cleavage of GSDMB, showcasing its involvement in the regulation of programmed cell death. Notably, ELANE exhibits bactericidal activity by effectively killing E. coli in vitro, while sparing S. aureus. This antimicrobial effect is attributed to its ability to digest outer membrane protein A (ompA) in E. coli and K. pneumoniae, underscoring its role in bacterial defense mechanisms.

Biological Activity

1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Measured by its ability to cleave the fluorogenic peptide substrate, MeOSuc-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin (MeOSuc-AAPV-AMC). The specific activity is 1776.266 pmol/min/µg, as measured under the described conditions.

Species

Human

Source

E. coli

Tag

N-GST

Accession

P08246 (I30-H267)

Gene ID
Molecular Construction
N-term
GST
ELANE (I30-H267)
Accession # P08246
C-term
Synonyms
Bone marrow serine protease; ELA2; ELANE; Elastase 2; Elastase 2 neutrophil; Elastase neutrophil expressed; Elastase-2; ELNE_HUMAN; GE; Granulocyte derived elastase; HLE; HNE; Human leukocyte elastase; Leukocyte elastase; Medullasin; NE; Neutrophil elastase; PMN E; PMN elastase; Polymorphonuclear elastase; SCN1
AA Sequence

IVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH

Molecular Weight

Approximately 52.6 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

ELANE Protein, Human (GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ELANE Protein, Human (GST)
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