EN1 Protein, Gallus gallus (His-B2M)
EN1 Protein is essential for the correct formation of the apical ectodermal ridge and accurate dorsal-ventral patterning in the limb. EN1 Protein, Gallus gallus (His-B2M) is the recombinant EN1 protein, expressed by E. coli , with N-6*His, B2M labeled tag.
- Species: Others
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Biological Activity
Description
EN1 Protein is essential for the correct formation of the apical ectodermal ridge and accurate dorsal-ventral patterning in the limb. EN1 Protein, Gallus gallus (His-B2M) is the recombinant EN1 protein, expressed by E. coli , with N-6*His, B2M labeled tag.
Background
EN1 plays a pivotal role in embryonic development, specifically contributing to the proper formation of the apical ectodermal ridge and ensuring accurate dorsal-ventral patterning in limb development.
Technical Parameters
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Species Others
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Source E. coli
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Tag N-6*His;B2M
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Accession
Q05916 (M1-E333)
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Gene ID771008 [NCBI]
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Molecular Construction
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N-term
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6*His-B2M
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EN1 (M1-333E)
Accession # Q05916 -
C-term
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Protein Length
Full Length
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Synonyms
EN1; Hu-En-1; Engrailed Homeobox 1; Engrailed Homolog 1; Homeobox Protein Engrailed-1; ENDOVESLB; Homeobox Protein En-1
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AA Sequence
MEEPPEGHGHHRDAAPPGPANGGGGGGGGSDGDSAPVSPSPAPASPAAPCPLPLPRRRPPPPPPPRTTNFFIDNILRPDFGCKKEPPAATGAATGAGGGGGGGGREQRDGAQSAGRENVNPLLARPPHAPSSALLCPDSNCAPDGSAPAGTAAKANPGTAAGAAGAAGAAKAQGDGGETPAAKYGEHGSPAILLMGSNNGGAVLKPDSQQPLVWPAWVYCTRYSDRPSSPRTRKLKKKKTEKEDKRPRTAFTAEQLQRLKAEFQANRYITEQRRQSLAQELSLNESRVKIWFQNKRAKIKKATGIKNGLALHLMAQGLYNHSTTTVQDKEESE
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Predicted Molecular Mass
48.5 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US;may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)