Endothelin-1/Edn1 Protein, Rat (His-KSI)

Endothelin-1/Edn1 protein is an endothelium-derived vasoconstrictor peptide that plays a crucial role in vascular regulation. As a possible ligand of the G protein-coupled receptors EDNRA and EDNRB, it initiates a signaling cascade involving PTK2B, BCAR1, BCAR3 and the GTPases RAP1 and RHOA in glomerular mesangial cells, helping to regulate vascular tone. Endothelin-1/Edn1 Protein, Rat (His-KSI) is the recombinant rat-derived Endothelin-1/Edn1 protein, expressed by E. coli , with N-6*His, N-KSI labeled tag. The total length of Endothelin-1/Edn1 Protein, Rat (His-KSI) is 21 a.a., with molecular weight of ~17.8 kDa.

Endothelin-1 (Edn1) is a vasoconstrictor peptide derived from endothelial cells, acting through G-protein coupled receptors EDNRA and EDNRB. It likely functions as a ligand for these receptors, activating downstream signaling cascades involving PTK2B, BCAR1, BCAR3, and GTPases RAP1 and RHOA in glomerular mesangial cells. Furthermore, Endothelin-1 binds to the DEAR/FBXW7-AS1 receptor. This intricate interaction highlights its role in mediating various cellular responses, particularly in the regulation of vascular tone and mesangial cell function. The multifaceted actions of Endothelin-1 underscore its significance in vascular physiology and suggest its involvement in diverse cellular processes (

Rat

E. coli

N-6*His;N-KSI

P22388 (53C-73W)

24323  [NCBI]

CSCSSLMDKECVYFCHLDIIW

Approximately 17.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.