ENTE Protein, S. aureus (N-His)

ENTE Proteinas are staphylococcal enterotoxins that activate the host immune system by binding to major histocompatibility complex class II and T-cell receptor (TCR) molecules. This interaction forms a ternary complex that activates T lymphocytes and induces the systemic release of proinflammatory cytokines. ENTE Protein, S. aureus (N-His) is the recombinant Staphylococcus aureus-derived ENTE protein, expressed by E. coli , with N-6*His labeled tag. The total length of ENTE Protein, S. aureus (N-His) is 230 a.a., with molecular weight of ~27 kDa.

ENTE, a staphylococcal enterotoxin, plays a pivotal role in activating the host immune system by binding as unprocessed molecules to major histocompatibility complex class II and T-cell receptor (TCR) molecules. This interaction results in the formation of a ternary complex, which, in turn, activates a significant number of T-lymphocytes, triggering a systemic release of pro-inflammatory cytokines. Beyond its immunomodulatory functions, ENTE is implicated in the development of staphylococcal food poisoning syndrome, a condition characterized by symptoms such as intoxication, fever, and gastrointestinal distress. These activities highlight the impact of ENTE on both immune response pathways and pathological outcomes.

Data is not available.

Staphylococcus aureus

E. coli

N-6*His

P12993 (S28-T257)

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SEEINEKDLRKKSELQRNALSNLRQIYYYNEKAITENKESDDQFLENTLLFKGFFTGHPWYNDLLVDLGSKDATNKYKGKKVDLYGAYYGYQCAGGTPNKTACMYGGVTLHDNNRLTEEKKVPINLWIDGKQTTVPIDKVKTSKKEVTVQELDLQARHYLHGKFGLYNSDSFGGKVQRGLIVFHSSEGSTVSYDLFDAQGQYPDTLLRIYRDNKTINSENLHIDLYLYTT

Approximately 27 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For lower concentration, please reconstitute in 50 mM Tris-HCL,300 mM NaCl, pH 7.4 buffer.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.