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  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Enterokinase Protein, Bovine (P.pastoris, His)

Enterokinase Protein, Bovine (P.pastoris, His)

Cat. No.: HY-P7341
COA Handling Instructions

Enterokinase Protein, Bovine (P.pastoris, His) is a single glycosylated polypeptide chain with His tag. Enterokinase plays a role of turning trypsinogen to its active form trypsin.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
10 μg $90 In-stock
50 μg $150 In-stock
100 μg $240 In-stock
> 100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

  • References

Description

Enterokinase Protein, Bovine (P.pastoris, His) is a single glycosylated polypeptide chain with His tag. Enterokinase plays a role of turning trypsinogen to its active form trypsin.

Background

Bovine enterokinase is a serine protease and functions as the physiological activator of trypsinogen. The amino acid composition shows that the light chain has nine half-cystine residues and that one half-cystine was in a disulfide link between the light and heavy subunits. The sequence has homologies with other serine proteases containing one or two chains. The homologies suggest that the catalytic subunit has the same three-dimensional structure and, therefore, the same mechanism of enzymatic action as pancreatic chymotrypsin, trypsin, and elastase. The presence of the conserved amino-terminal activation peptide sequence (IVGG) shows that enterokinase must have a zymogen precursor and that the two-chain enzyme arises from limited proteolysis during posttranslational processing[1].

Biological Activity

100 IU/μg Unit Definition: One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25mM Tris-HCl, pH 8.0.

Species

Bovine

Source

P. pastoris

Tag

C-His

Accession

/

Gene ID

/

Synonyms
rBoEnterokinase, His; Enteropeptidase; ENTK; PRSS7
Molecular Weight

Approximately 40 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized after extensive dialysis against 20 mM Tris-HCl, pH 7.4, 200 mM NaCl, 2 mM CaCl2.

Endotoxin Level

<0.2 EU/μg, determined by LAL method.

Reconstitution

Reconstitute at 1 mg/mL in sterile deionied water, then dilute in sterile EK Storage Buffer (20 mM Tris-HCl, pH 7.4, 200 mM NaCl, 2 mM CaCl2).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

Enterokinase Protein, Bovine (P.pastoris, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Enterokinase Protein, Bovine (P.pastoris, His)
Cat. No.:
HY-P7341
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