1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Enterokinase Protein, Bovine (P.pastoris, His)

Enterokinase Protein, Bovine (P.pastoris, His)

Cat. No.: HY-P7341
Handling Instructions

Enterokinase Protein, Bovine (P.pastoris, His) is a single glycosylated polypeptide chain with His tag. Enterokinase plays a role of turning trypsinogen to its active form trypsin.

For research use only. We do not sell to patients.

Protein Expression Service

Recombinant Proteins-MedChemExpress
Size Price Stock Quantity
10 μg $90 In-stock
Estimated Time of Arrival: December 31
50 μg $150 In-stock
Estimated Time of Arrival: December 31
100 μg   Get quote  
250 μg   Get quote  

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Enterokinase Protein, Bovine (P.pastoris, His) Related Classifications

  • Technical Parameters

  • Properties

  • Documentation

Species

Bovine

Source

P. pastoris

Tag

C-His

Accession

/

Synonyms
rBoEnterokinase, His; Enteropeptidase; ENTK; PRSS7
AA Sequence

Molecular Weight

Approximately 40 kDa

Biological Activity
100 IU/μg
Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against 20 mM Tris-HCl, pH 7.4, 200 mM NaCl, 2 mM CaCl2.

Endotoxin Level

<0.2 EU/μg, determined by LAL method.

Reconstitution

Reconstitute at 1 mg/mL in sterile deionied water, then dilute in sterile EK Storage Buffer (20 mM Tris-HCl, pH 7.4, 200 mM NaCl, 2 mM CaCl2).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Background

Bovine enterokinase is a serine protease and functions as the physiological activator of trypsinogen. The amino acid composition shows that the light chain has nine half-cystine residues and that one half-cystine was in a disulfide link between the light and heavy subunits. The sequence has homologies with other serine proteases containing one or two chains. The homologies suggest that the catalytic subunit has the same three-dimensional structure and, therefore, the same mechanism of enzymatic action as pancreatic chymotrypsin, trypsin, and elastase. The presence of the conserved amino-terminal activation peptide sequence (IVGG) shows that enterokinase must have a zymogen precursor and that the two-chain enzyme arises from limited proteolysis during posttranslational processing[1].

Documentation
References
  • Reconstitution Calculator

  • Dilution Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
Enterokinase Protein, Bovine (P.pastoris, His)
Cat. No.:
HY-P7341
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