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ERG11 Protein, Saccharomyces cerevisiae (GST)

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ERG11 Proteinas is a sterol 14α-demethylase, a key enzyme in the ergosterol biosynthetic pathway, particularly in the late pathway that occurs in the endoplasmic reticulum membrane. ERG11 catalyzes the three-step removal of the 14α-methyl group from lanosterol, releasing it as a formate, resulting in the conversion of the sterol into a key intermediate in ergosterol biosynthesis. ERG11 Protein, Saccharomyces cerevisiae (GST) is the recombinant ERG11 protein, expressed by E. coli , with N-GST labeled tag.

For research use only. We do not sell to patients.
  • Species: Others
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • ERG11 Protein, Saccharomyces cerevisiae (GST) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

ERG11 Proteinas is a sterol 14α-demethylase, a key enzyme in the ergosterol biosynthetic pathway, particularly in the late pathway that occurs in the endoplasmic reticulum membrane. ERG11 catalyzes the three-step removal of the 14α-methyl group from lanosterol, releasing it as a formate, resulting in the conversion of the sterol into a key intermediate in ergosterol biosynthesis. ERG11 Protein, Saccharomyces cerevisiae (GST) is the recombinant ERG11 protein, expressed by E. coli , with N-GST labeled tag.

Background

ERG11 protein, a sterol 14alpha-demethylase, is a crucial enzyme in the third module of the ergosterol biosynthesis pathway, playing a pivotal role in fungal membranes where ergosterol serves as the major sterol component with diverse functions. The third module, known as the late pathway, involves consecutive reactions primarily occurring in the endoplasmic reticulum (ER) membrane, ultimately leading to ergosterol synthesis. Initiating from lanosterol, ERG11 catalyzes a three-step oxidative removal of the 14alpha-methyl group (C-32) of the sterol, releasing it in the form of formate. This process results in the conversion of the sterol to 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol, a critical intermediate in ergosterol biosynthesis. Interestingly, ERG11 exhibits the ability to demethylate substrates not intrinsic to yeast, including eburicol and 24,25-dihydrolanosterol, highlighting its versatility in sterol modification.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Others
  • Source E. coli
  • Tag N-GST
  • Accession

    P10614 (M1-S20)

  • Gene ID
    856398  [NCBI]
  • Molecular Construction
    • N-term
    • GST
    • ERG11 (M1-S20)
      Accession # P10614
    • C-term
  • Protein Length

    Lumenal Domain

  • Synonyms

    Lanosterol 14-alpha demethylase CYP51; EC:1.14.14.154; Cytochrome P450 51; Cytochrome P450-14DM; Cytochrome P450-LIA1; CYPLI; Ergosterol biosynthetic protein 11; Lanosterol C14-C32 lyase; Sterol 14-alpha demethylase; ERG11; CYP51; YHR007C

  • AA Sequence

    MSATKSIVGEALEYVNIGLS

  • Predicted Molecular Mass

    29.1 kDa

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • ERG11 Protein, Saccharomyces cerevisiae (GST) Product Image