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  4. FAM20B Protein, Human (HEK293, Fc)

FAM20B Protein, Human (HEK293, Fc)

Cat. No.: HY-P76326
SDS COA Handling Instructions

The FAM20B protein plays a key role in regulating mature glycosaminoglycan (GAG) chains by catalyzing xylose 2-O-phosphorylation in the glycosaminoglycan-protein linking region of the proteoglycan. This process is critical for the synthesis of sulfated GAGs (such as heparan sulfate and chondroitin sulfate) on the common GAG-protein linking region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of the core protein. FAM20B Protein, Human (HEK293, Fc) is the recombinant human-derived FAM20B protein, expressed by HEK293 , with N-hFc labeled tag. The total length of FAM20B Protein, Human (HEK293, Fc) is 379 a.a., with molecular weight of ~75-80 KDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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  • Documentation

Description

The FAM20B protein plays a key role in regulating mature glycosaminoglycan (GAG) chains by catalyzing xylose 2-O-phosphorylation in the glycosaminoglycan-protein linking region of the proteoglycan. This process is critical for the synthesis of sulfated GAGs (such as heparan sulfate and chondroitin sulfate) on the common GAG-protein linking region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of the core protein. FAM20B Protein, Human (HEK293, Fc) is the recombinant human-derived FAM20B protein, expressed by HEK293 , with N-hFc labeled tag. The total length of FAM20B Protein, Human (HEK293, Fc) is 379 a.a., with molecular weight of ~75-80 KDa.

Background

The FAM20B protein assumes a pivotal role in the regulation of mature glycosaminoglycan (GAG) chains by catalyzing the 2-O-phosphorylation of xylose within the glycosaminoglycan-protein linkage region of proteoglycans. This process is integral to the synthesis of sulfated GAGs, including heparan sulfate and chondroitin sulfate, on the common GAG-protein linkage region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of core proteins. The stepwise addition of monosaccharide residues by specific glycosyltransferases forms this linkage region, where xylose 2-O-phosphorylation plays a regulatory role in the catalytic activity of B3GAT3 (GlcAT-I). B3GAT3 completes the precursor tetrasaccharide of GAG-protein linkage regions, serving as a foundation for the subsequent synthesis of the repeating disaccharide region. FAM20B's involvement in xylose 2-O-phosphorylation highlights its crucial contribution to the precise regulation of GAG chain assembly and the intricate orchestration of glycosaminoglycan structures.

Biological Activity

Measured by its ability to enhance survival of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 1.464 μg/mL. Corresponding to a specific activity is 683.06 unit/mg.

  • Measured by its ability to enhance survival of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 1.464 μg/mL. Corresponding to a specific activity is 683.06 Unit/mg.
Species

Human

Source

HEK293

Tag

N-hFc

Accession

O75063/NP_055679.1 (S31-L409)

Gene ID
Molecular Construction
N-term
hFc
FAM20B (S31-L409)
Accession # O75063/NP_055679.1
C-term
Synonyms
Glycosaminoglycan xylosylkinase; Xylose kinase; KIAA0475
AA Sequence

SAANREDQRAFHRMMTGLRVELAPKLDHTLQSPWEIAAQWVVPREVYPEETPELGAVMHAMATKKIIKADVGYKGTQLKALLILEGGQKVVFKPKRYSRDHVVEGEPYAGYDRHNAEVAAFHLDRILGFHRAPLVVGRFVNLRTEIKPVATEQLLSTFLTVGNNTCFYGKCYYCRETEPACADGDIMEGSVTLWLPDVWPLQKHRHPWGRTYREGKLARWEYDESYCDAVKKTSPYDSGPRLLDIIDTAVFDYLIGNADRHHYESFQDDEGASMLILLDNAKSFGNPSLDERSILAPLYQCCIIRVSTWNRLNYLKNGVLKSALKSAMAHDPISPVLSDPHLDAVDQRLLSVLATVKQCTDQFGMDTVLVEDRMPLSHL

Molecular Weight

Approximately 75-80 kDa due to the glycosylation

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

FAM20B Protein, Human (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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FAM20B Protein, Human (HEK293, Fc)
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HY-P76326
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