FAM20C Protein, Human (HEK293, Myc, His)

The FAM20C protein is a Golgi kinase that phosphorylates secretory pathway proteins and contributes to the biomineralization of bones and teeth. FAM20C phosphorylates caseins and proteins involved in biomineralization and also affects lipid homeostasis, wound healing, and cell migration and adhesion. FAM20C Protein, Human (HEK293, Myc, His) is the recombinant human-derived FAM20C protein, expressed by HEK293 , with N-His, C-Myc labeled tag. The total length of FAM20C Protein, Human (HEK293, Myc, His) is 492 a.a., with molecular weight of ~61.4 kDa.

The FAM20C Protein functions as a Golgi serine/threonine protein kinase with a pivotal role in the phosphorylation of secretory pathway proteins within Ser-x-Glu/pSer motifs, crucial for the biomineralization of bones and teeth. Serving as the primary protein kinase for extracellular proteins, FAM20C significantly contributes to the generation of the extracellular phosphoproteome, displaying a preference for proteins within the Ser-x-Glu/pSer motif while also exhibiting broader substrate specificity. Notably, FAM20C phosphorylates ERO1A to enhance its activity, crucial for maintaining endoplasmic reticulum redox homeostasis and facilitating oxidative protein folding. In times of endoplasmic reticulum stress, FAM20C phosphorylates P4HB/PDIA1, inducing a functional switch that transforms P4HB from an oxidoreductase to a molecular chaperone, crucial for maintaining ER proteostasis and reducing cell death under ER stress. Additionally, FAM20C is essential for osteoblast differentiation and mineralization, phosphorylating various proteins involved in biomineralization, such as AMELX, AMTN, ENAM, and SPP1/OPN. Beyond its role in biomineralization, FAM20C also contributes to lipid homeostasis, wound healing, and cell migration and adhesion.

Human

HEK293

N-His;C-Myc

Q8IXL6 (93D-584R)

56975  [NCBI]

DFSSDPSSNLSSHSLEKLPPAAEPAERALRGRDPGALRPHDPAHRPLLRDPGPRRSESPPGPGGDASLLARLFEHPLYRVAVPPLTEEDVLFNVNSDTRLSPKAAENPDWPHAGAEGAEFLSPGEAAVDSYPNWLKFHIGINRYELYSRHNPAIEALLHDLSSQRITSVAMKSGGTQLKLIMTFQNYGQALFKPMKQTREQETPPDFFYFSDYERHNAEIAAFHLDRILDFRRVPPVAGRMVNMTKEIRDVTRDKKLWRTFFISPANNICFYGECSYYCSTEHALCGKPDQIEGSLAAFLPDLSLAKRKTWRNPWRRSYHKRKKAEWEVDPDYCEEVKQTPPYDSSHRILDVMDMTIFDFLMGNMDRHHYETFEKFGNETFIIHLDNGRGFGKYSHDELSILVPLQQCCRIRKSTYLRLQLLAKEEYKLSLLMAESLRGDQVAPVLYQPHLEALDRRLRVVLKAVRDCVERNGLHSVVDDDLDTEHRAASAR

Approximately 61.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.