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  3. FAM3D Protein, Mouse (223a.a, HEK293, Fc)

FAM3D Protein, Mouse (223a.a, HEK293, Fc)

Cat. No.: HY-P76328
COA Handling Instructions

FAM3D Protein, part of the FAM3 family, serves as a transmembrane ligand within the ephrin family, crucial in cellular signaling. Interacting with its Eph receptor, FAM3D initiates bidirectional signaling, regulating diverse cellular processes in development and implicated in tissue boundary formation, axon guidance, angiogenesis, and synaptic plasticity. Linked to pathological conditions like cancer, cardiovascular diseases, and neurological disorders, FAM3D emerges as a potential therapeutic target. FAM3D Protein, Mouse (223a.a, HEK293, Fc) is the recombinant mouse-derived FAM3D protein, expressed by HEK293, with C-hFc labeled tag. The total length of FAM3D Protein, Mouse (223a.a, HEK293, Fc) is 198 a.a., with molecular weight of ~54 & 29 KDa, respectively.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

FAM3D Protein, part of the FAM3 family, serves as a transmembrane ligand within the ephrin family, crucial in cellular signaling. Interacting with its Eph receptor, FAM3D initiates bidirectional signaling, regulating diverse cellular processes in development and implicated in tissue boundary formation, axon guidance, angiogenesis, and synaptic plasticity. Linked to pathological conditions like cancer, cardiovascular diseases, and neurological disorders, FAM3D emerges as a potential therapeutic target. FAM3D Protein, Mouse (223a.a, HEK293, Fc) is the recombinant mouse-derived FAM3D protein, expressed by HEK293, with C-hFc labeled tag. The total length of FAM3D Protein, Mouse (223a.a, HEK293, Fc) is 198 a.a., with molecular weight of ~54 & 29 KDa, respectively.

Background

FAM3D Protein belongs to the FAM3 family. It is a member of the ephrin family, which consists of proteins that play crucial roles in cellular signaling and communication. FAM3D specifically functions as a transmembrane ligand, interacting with its corresponding Eph receptor to initiate bidirectional signaling events that regulate a wide range of cellular processes. This protein is involved in various developmental processes such as tissue boundary formation, axon guidance, angiogenesis, and synaptic plasticity. Furthermore, FAM3D has been implicated in several pathological conditions, including cancer, cardiovascular diseases, and neurological disorders, thereby making it a potential target for therapeutic intervention.

Species

Mouse

Source

HEK293

Tag

C-hFc

Accession

P97805 (Y26-M223)

Gene ID

18300  [NCBI]

Molecular Construction
N-term
FAM3D (Y26-M223)
Accession # P97805
hFc
C-term
Synonyms
Protein FAM3D; FAM3D; Protein EF-7; OIT1
Molecular Weight

Approximately 54&29 kDa.

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

FAM3D Protein, Mouse (223a.a, HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
FAM3D Protein, Mouse (223a.a, HEK293, Fc)
Cat. No.:
HY-P76328
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