Flagellin Protein, Listeria monocytogenes (His)

Flagellin Protein is the subunit protein which polymerizes to form the filaments of bacterial flagella.It is a natural immune agonist that activates innate immunity and then stimulates the adaptive immune response.Flagellin directly activates a number of immune and non-immune cells through TLR5.In addition, it has anti-tumor and radioprotective activity and shows great potential in combating tumor growth and radiation-related tissue damage. Flagellin Protein, Listeria monocytogenes (His) is the recombinant Flagellin protein, expressed by E.coli , with N-6*His labeled tag.

Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. Flagellin is a natural immune agonist that activates innate immunity and then stimulates the adaptive immune response. It is expressed in many types of immune cells, including epithelial cells, polymorphonuclear neutrophils, natural killer (NK) cells, myeloid dendritic cells (mDCs), monocytes, Langerhans cells, macrophages and T cells. Flagellin, as an adjuvant, can induce an enhanced antigen-specific immune response. Flagellin directly activates a number of immune and non-immune cells, including T, B, DCs, NK and non-lymphoid cells (macrophages, epithelial, fibroblasts, stromal cells and neutrophils), through TLR5. The cumulative effect of this activation is the augmentation of immune responses through the generation of more potent antibodies and a Th1 response. In addition, it has anti-tumor and radioprotective activity and shows great potential in combating tumor growth and radiation-related tissue damage^[1][2].

Immobilized Flagellin Protein at 5 μg/mL (100 μL/well) can bind Anti-Flagellin antibody. The ED₅₀ for this effect is 30-100 ng/mL.

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  Immobilized Flagellin Protein at 5 μg/mL (100 μL/well) can bind Anti-Flagellin antibody. The ED₅₀ for this effect is 60.34 ng/mL.

Others

E. coli

N-6*His

WP_003724427.1 (M1-S287)

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Full Length

MKVNTNIISLKTQEYLRKNNEGMTQAQERLASGKRINSSLDDAAGLAVVTRMNVKSTGLDAASKNSSMGIDLLQTADSALSSMSSILQRMRQLAVQSSNGSFSDEDRKQYTAEFGSLIKELDHVADTTNYNNIKLLDQTATGAATQVSIQASDKANDLINIDLFNAKGLSAGTITLGSGSTVAGYSALSVADADSSQEATEAIDELINNISNGRALLGAGMSRLSYNVSNVNNQSIATKASASSIEDADMAAEMSEMTKYKILTQTSISMLSQANQTPQMLTQLINS

Approximately 33 kDa

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of PBS.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.