GLUT1 Protein, Human (sf9, His, N45T)

GLUT1 Protein, Human (sf9, His, N45T) is the recombinant human-derived GLUT1, expressed by sf9 insect cells , with His labeled tag. ,

Glucose Transporter GLUT1 is a facilitative glucose transporter responsible for constitutive or basal glucose uptake. It exhibits broad substrate specificity, capable of transporting various aldoses, including both pentoses and hexoses. As the primary energy carrier in the brain, GLUT1 is present at the blood-brain barrier and mediates energy-independent, facilitative glucose transport into the brain. In association with BSG and NXNL1, it promotes retinal cone survival by enhancing glucose uptake in photoreceptors (by similar). Additionally, it is required for mesendoderm differentiation (by similar).

Human

Sf9 insect cells

His

P11166 (M1-V492, N45T)

6513

Full Length

MEPSSKKLTGRLMLAVGGAVLGSLQFGYNTGVINAPQKVIEEFYTQTWVHRYGESILPTTLTTLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLMMNLLAFVSAVLMGFSKLGKSFEMLILGRFIIGVYCGLTTGFVPMYVGEVSPTALRGALGTLHQLGIVVGILIAQVFGLDSIMGNKDLWPLLLSIIFIPALLQCIVLPFCPESPRFLLINRNEENRAKSVLKKLRGTADVTHDLQEMKEESRQMMREKKVTILELFRSPAYRQPILIAVVLQLSQQLSGINAVFYYSTSIFEKAGVQQPVYATIGSGIVNTAFTVVSLFVVERAGRRTLHLIGLAGMAGCAILMTIALALLEQLPWMSYLSIVAIFGFVAFFEVGPGPIPWFIVAELFSQGPRPAAIAVAGFSNWTSNFIVGMCFQYVEQLCGPYVFIIFTVLLVLFFIFTYFKVPETKGRTFDEIASGFRQGGASQSDKTPEELFHPLGADSQV

≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.002%, w/v LMNG.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.