GPR158 Protein, Human (HEK293, GFP, FLAG, His)

The GPR158 protein is a key orphan receptor in vision that regulates signaling in retinal depolarizing bipolar cells. As an atypical G protein-coupled receptor, it recruits and regulates the R7 group RGS-GNB5 complex, which is different from traditional G protein activation. GPR158 Protein, Human (HEK293, GFP, FLAG, His) is the recombinant human-derived GPR158 protein, expressed by HEK293 , with C-10*His, C-GFP, C-Flag labeled tag. The total length of GPR158 Protein, Human (HEK293, GFP, FLAG, His) is 775 a.a., .

GPR158 Protein, an orphan receptor integral to vision, plays a crucial role in signal transduction through retinal depolarizing bipolar cells. Functioning as an atypical G-protein coupled receptor, it recruits and regulates R7 group RGS-GNB5 complexes, diverging from conventional G protein activation. GPR158 promotes the GTPase activator activity of R7 RGS proteins, leading to the inactivation of G protein alpha subunits. This regulatory mechanism influences components of the metabotropic signaling cascade in retina ON-bipolar neurons, including TRPM1 and GRM6. GPR158 forms homodimers and associates with R7 group RGS-GNB5 complexes, thereby facilitating their membrane localization and modulating the GTPase activator activity of R7 RGS proteins. Interactions with TRPM1 and GRM6 suggest its involvement in controlling the GRM6 cascade's ability to gate TRPM1. Furthermore, GPR158 engages in a transsynaptic interaction with EGFLAM, emphasizing its role in synaptic organization of photoreceptor cells.

Human

HEK293

C-10*His;C-GFP;C-Flag

Q6PRD1 (M1-E775)

440435

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.