1. Recombinant Proteins
  2. Receptor Proteins
  3. G-Protein-Coupled Receptors (GPCRs)
  4. GPR35 Protein, Human (Cell-Free, His)

GPR35 Protein, Human (Cell-Free, His)

Cat. No.: HY-P702306
Handling Instructions

GPR35 Protein, a receptor for kynurenic acid, operates through G(qi/o) proteins, triggering calcium mobilization and inositol phosphate production. It plays a pivotal role in transducing signals related to tryptophan metabolism, contributing to cellular responses via intricate G-protein signaling pathways. GPR35 Protein, Human (Cell-Free, His) is the recombinant human-derived GPR35 protein, expressed by E. coli Cell-free, with N-10*His labeled tag. The total length of GPR35 Protein, Human (Cell-Free, His) is 309 a.a., with molecular weight of 36.9 kDa.

For research use only. We do not sell to patients.

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Description

GPR35 Protein, a receptor for kynurenic acid, operates through G(qi/o) proteins, triggering calcium mobilization and inositol phosphate production. It plays a pivotal role in transducing signals related to tryptophan metabolism, contributing to cellular responses via intricate G-protein signaling pathways. GPR35 Protein, Human (Cell-Free, His) is the recombinant human-derived GPR35 protein, expressed by E. coli Cell-free, with N-10*His labeled tag. The total length of GPR35 Protein, Human (Cell-Free, His) is 309 a.a., with molecular weight of 36.9 kDa.

Background

GPR35 Protein operates as a receptor for kynurenic acid, a key intermediate in the tryptophan metabolic pathway. Its functional activity is orchestrated by G-proteins, specifically triggering calcium mobilization and inositol phosphate production through G(qi/o) proteins. In this capacity, GPR35 plays a pivotal role in transducing signals related to tryptophan metabolism, contributing to cellular responses mediated by intricate G-protein signaling pathways.

Species

Human

Source

E. coli Cell-free

Tag

N-10*His

Accession

Q9HC97 (M1-A309)

Gene ID

2859

Molecular Construction
N-term
10*His
GPR35 (M1-A309)
Accession # Q9HC97
C-term
Synonyms
G-protein coupled receptor 35; Kynurenic acid receptor; KYNA receptor
AA Sequence

MNGTYNTCGSSDLTWPPAIKLGFYAYLGVLLVLGLLLNSLALWVFCCRMQQWTETRIYMTNLAVADLCLLCTLPFVLHSLRDTSDTPLCQLSQGIYLTNRYMSISLVTAIAVDRYVAVRHPLRARGLRSPRQAAAVCAVLWVLVIGSLVARWLLGIQEGGFCFRSTRHNFNSMAFPLLGFYLPLAVVVFCSLKVVTALAQRPPTDVGQAEATRKAARMVWANLLVFVVCFLPLHVGLTVRLAVGWNACALLETIRRALYITSKLSDANCCLDAICYYYMAKEFQEASALAVAPSAKAHKSQDSLCVTLA

Molecular Weight

36.9 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

GPR35 Protein, Human (Cell-Free, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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GPR35 Protein, Human (Cell-Free, His)
Cat. No.:
HY-P702306
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