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  4. Gremlin-1 Protein, Mouse/Rat (HEK293, His, Myc)

Gremlin-1 Protein, Mouse/Rat (HEK293, His, Myc)

Cat. No.: HY-P705912
Handling Instructions Technical Support

Gremlin-1 Protein, Mouse/Rat (HEK293, His, Myc) is the recombinant human-derived Gremlin-1 protein, expressed by HEK293, with C-His tag.

For research use only. We do not sell to patients.

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Description

Gremlin-1 Protein, Mouse/Rat (HEK293, His, Myc) is the recombinant human-derived Gremlin-1 protein, expressed by HEK293, with C-His tag.
Background

Gremlin-1 is a cytokine potentially involved in carcinogenesis and metanephric kidney organogenesis. Functioning as a BMP antagonist, it is essential for early limb outgrowth and patterning by maintaining the FGF4-SHH feedback loop. It downregulates BMP4 signaling in a dose-dependent manner (By similarity). As an antagonist of BMP2, it inhibits BMP2-mediated osteoblast differentiation (in vitro). Additionally, it acts as an inhibitor of monocyte chemotaxis. Overexpression can suppress the growth/viability of normal cells but not transformed cells (By similarity).
Species

Rat; Mouse
Source

HEK293
Tag

N-10*His;C-Myc
Accession

O70326/O35793 (K25-D184)
Gene ID

23892/50566
Molecular Construction
N-term
10*His
(K25-D184)
Accession # O70326/O35793
Myc
C-term
Protein Length

Full Length of Mature Protein
Synonyms
GREM1,CKTSF1B1,DAND2,DRM,PIG2
AA Sequence

KKKGSQGAIPPPDKAQHNDSEQTQSPPQPGSRTRGRGQGRGTAMPGEEVLESSQEALHVTERKYLKRDWCKTQPLKQTIHEEGCNSRTIINRFCYGQCNSFYIPRHIRKEEGSFQSCSFCKPKKFTTMMVTLNCPELQPPTKKKRVTRVKQCRCISIDLD

Predicted Molecular Mass
22.2 kDa
Purity

≥ 90%, as determined by reducing SDS-PAGE.
Documentation
Handling Instructions (2659 KB)

Gremlin-1 Protein, Mouse/Rat (HEK293, His, Myc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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