2. Recombinant Proteins
  3. Viral Proteins
  4. Influenza Viruses Proteins
  5. Influenza Hemagglutinin
  6. HA/Hemagglutinin Protein, H1N1 (ABD77807, sf9, His)

HA/Hemagglutinin Protein, H1N1 (ABD77807, sf9, His)

Cat. No.: HY-P74080
Instrucciones de manejo Technical Support

The hemagglutinin (HA) protein attaches viral particles to host cells by binding to sialic acid-containing receptors and induces viral particle internalization through clathrin-dependent endocytosis.HA also promotes an alternative clathrin- and caveolin-independent pathway for some virions.HA/Hemagglutinin Protein, H1N1 (ABD77807, sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag.

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Descripciòn

The hemagglutinin (HA) protein attaches viral particles to host cells by binding to sialic acid-containing receptors and induces viral particle internalization through clathrin-dependent endocytosis.HA also promotes an alternative clathrin- and caveolin-independent pathway for some virions.HA/Hemagglutinin Protein, H1N1 (ABD77807, sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag.
Background

The Hemagglutinin (HA) protein plays a crucial role in the attachment of virus particles to host cells by binding to sialic acid-containing receptors on the cell surface. This interaction not only induces virion internalization through clathrin-dependent endocytosis but also facilitates an alternative clathrin- and caveolin-independent pathway for about one-third of the virus particles. HA is a Class I viral fusion protein responsible for penetrating the cell cytoplasm by mediating the fusion of the virus particle's membrane with the endosomal membrane. The low pH environment in endosomes triggers an irreversible conformational change in HA2, leading to the release of the fusion hydrophobic peptide. The cooperative action of several trimers is necessary to form a competent fusion pore, highlighting the intricate role of HA in host range restriction and virulence determination.
Species

Virus
Source

Sf9 insect cells
Tag

C-His
Accession

ABD77807 (D18-Q529)
Gene ID

/
Molecular Construction
N-term
HA/Hemagglutinin (D18-Q529)
Accession # ABD77807
His
C-term
Protein Length

Partial
Synonyms
HA; Hemagglutinin; HA/Hemagglutinin Protein, H1N1 (A/Fort Monmouth/1/1947, sf9, His)
AA Sequence

DTICIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDSHNGKLCRLKGIAPLQLGKCNIAGWILGNPECESLLSKRSWSYIAETPNSENGTCYPGDFADYEELREQLSSVSSFERFEIFPKERSWPKHNITRGVTAACSHAGKSSFYKNLLWLTETNGSYPKLSKSYVNNKEKEVLVLWGVHHPSNIEDQKTLYRKENAYVSVVSSNYNRRFTPEIAERPKVRGQAGRMNYYWTLLEPGDTIIFEANGNLIAPWYAFALSRGFGSGIITSNASMDECDTKCQTPQGAINSSLPFQNIHPVTIGECPKYVKSTKLRMVTGLRNIPSIQSRGLFGAIAGFIEGGWTGMIDGWYGYHHQNEQGSGYAADQKSTQNAINGITNKVNSVIEKMNTQFTAVGKEFNKLEKRMENLNKKVDDGFLDIWTYNAELLVLLENERTLDFHDSNVKNLYEKVKNQLRNNAKEIGNGCFEFYHKCNNECMESVKNGTYDYPKYSEESKLNREKIDGVKLESMGVYQ

Predicted Molecular Mass
59.3 kDa
Pureza

≥ 90%, as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentación
Instrucciones de manejo (2659 KB)

HA/Hemagglutinin Protein, H1N1 (ABD77807, sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HA/Hemagglutinin Protein, H1N1 (ABD77807, sf9, His)
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