HA1/Hemagglutinin Protein, H1N1 (P03453, HEK293, His)

The HA/Hemagglutinin protein binds receptors on the cell surface, facilitating virus attachment.It leads to internalization via endocytosis.HA determines host range and virulence, mediating fusion of virus and endosomal membrane.HA2 undergoes conformational change in endosomes, releasing fusion peptide and forming fusion pore.Multiple HA trimers are required for pore formation.HA/Hemagglutinin Protein, H1N1 (P03453, HEK293, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by HEK293 , with C-His labeled tag.

The HA/Hemagglutinin protein binds to sialic acid-containing receptors on the cell surface, facilitating the attachment of the virus particle to the cell. This attachment leads to the internalization of approximately two thirds of the virus particles through clathrin-dependent endocytosis, while the remaining one third follows a clathrin- and caveolin-independent pathway. The protein plays a crucial role in determining the host range restriction and virulence. As a class I viral fusion protein, it is responsible for the penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. In the low pH environment of endosomes, HA2 undergoes an irreversible conformational change, resulting in the release of the fusion hydrophobic peptide and the formation of a competent fusion pore. Multiple trimers of HA are necessary for the formation of this pore.

Virus

HEK293

C-His

P03453 (D18-R344)

/

Full Length of HA1 chain

DTICIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDSHNGKLCRLKGIAPLQLGKCNIAGWILGNPECESLFSKKSWSYIAETPNSENGTCYPGYFADYEELREQLSSVSSFERFEIFPKERSWPKHNVTRGVTASCSHKGKSSFYRNLLWLTEKNGSYPNLSKSYVNNKEKEVLVLWGVHHPSNIEDQKTIYRKENAYVSVVSSNYNRRFTPEIAERPKVRGQAGRINYYWTLLEPGDTIIFEANGNLIAPWHAFALNRGFGSGIITSNASMDECDTKCQTPQGAINSSLPFQNIHPVTIGECPKYVRSTKLRMVTGLRNIPSIQSR

≥ 90%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.