HA/Hemagglutinin Protein, H5N1 (AAT76166, sf9, His)

HA/Hemagglutinin Protein is a viral membrane protein responsible for the initial steps of the entry of influenza virus into the host cell.It mediates binding of the virus particle to the host-cell membrane and catalyzes fusion of the viral membrane with that of the host.HA is pivotal in determining virus host range and virulence.HA/Hemagglutinin Protein, H5N1 (AAT76166, sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag.

HA (Hemagglutinin), a class I viral fusion protein, binds to sialic acid-containing receptors, initiating virus attachment to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. HA is pivotal in determining virus host range and virulence. Following endocytosis, HA mediates fusion of the virus and endosomal membranes, allowing virus entry into the cell cytoplasm. In the acidic endosomal environment, HA2 undergoes conformational changes, releasing a fusion peptide and forming a fusion pore. HA, existing as a homotrimer, comprises disulfide-linked HA1-HA2 subunits and interacts with human CACNA1C^[1][2][3].

Virus

Sf9 insect cells

C-His

AAT76166 (D17-Q531, with cleavage site mutated RERRRKKR to TETR)

/

Partial

Approximately 59.4 kDa

≥ 95%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.