HA/Hemagglutinin Protein, H5N1 (H8PF45, HA1, HEK293, His)

HA/Hemagglutinin Protein is pivotal in the viral life cycle, binding to sialic acid receptors and facilitating virus attachment to host cells. This interaction prompts virion internalization through clathrin-dependent or -independent pathways. As a Class I viral fusion protein, it mediates fusion between the virus particle and endosomal membrane, allowing cytoplasmic entry. In endosomes, the low pH induces an irreversible conformational change in HA2, releasing a fusion hydrophobic peptide. Multiple HA protein trimers are essential for forming a competent fusion pore. Its roles in receptor binding, viral internalization, fusion, and membrane penetration are crucial in determining host range restriction and virulence. HA/Hemagglutinin Protein, H5N1 (H8PF45, HA1, HEK293, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by HEK293, with C-His labeled tag. The total length of HA/Hemagglutinin Protein, H5N1 (H8PF45, HA1, HEK293, His) is 340 a.a., with molecular weight of ~38.1 kDa.

The HA protein, also known as Hemagglutinin Protein, plays a crucial role in the viral life cycle. It binds to cell surface receptors that contain sialic acid, facilitating the attachment of the virus particle to the host cell. This attachment leads to the internalization of the virion through either a clathrin-dependent endocytosis pathway or a clathrin- and caveolin-independent pathway. The HA protein is a Class I viral fusion protein, responsible for mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. This fusion event allows the virus to enter the cytoplasm of the cell. The low pH environment in the endosomes triggers an irreversible conformational change in the HA2 subunit of the protein, leading to the release of a fusion hydrophobic peptide. Multiple trimers of the HA protein are necessary to form a competent fusion pore. The HA protein's functions in receptor binding, viral internalization, fusion, and membrane penetration are critical for determining the host range restriction and virulence of the virus.

Virus

HEK293

C-His

H8PF45 (M1-E340)

/

Approximately 38.1 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.