HA1/Hemagglutinin Protein, H5N1 (Q692M2, HEK293, His)

The HA/hemagglutinin protein binds to sialic acid receptors, initiating viral attachment to cells. This binding promotes virion internalization via clathrin-dependent or -independent pathways. HA/Hemagglutinin Protein, H5N1 (Q692M2, HEK293, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by HEK293 , with C-His labeled tag.

The HA/Hemagglutinin Protein binds to sialic acid-containing receptors on the cell surface, facilitating the attachment of the virus particle to the cell. This attachment triggers virion internalization through either clathrin-dependent endocytosis or a clathrin- and caveolin-independent pathway. It plays a crucial role in determining the host range restriction and virulence of the virus. As a Class I viral fusion protein, it is responsible for mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane, allowing the penetration of the virus into the cell cytoplasm. The low pH environment in endosomes causes an irreversible conformational change in HA2, leading to the release of the fusion hydrophobic peptide. Multiple trimers are necessary to form a competent fusion pore.

Virus

HEK293

C-His

Q692M2 (D17-R342)

/

Partial

DQICIGYHANNSTEQVDTIMEKNVTVTHAQDILEKTHNGKLCDLDGVKPLILRDCSVAGWLLGNPMCDEFINVPEWSYIVEKASPANDLCYPGDFNDYEELKHLLSRINHFEKIQIIPKSSWSNHEASSGVSSACPYQGKSSFFRNVVWLIKKNSTYPTIKRSYNNTNQEDLLVLWGIHHPNDAAEQTKLYQNPTTYISVGTSTLNQRLVPKIATRSKVNGQSGRMEFFWTILKPNDAINFESNGNFIAPEYAYKIVKKGDSAIMKSELEYGNCNTKCQTPMGAINSSMPFHNIHPLTIGECPKYVKSNRLVLATGLRNSPQRERR

≥ 95%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.