HCMV Glycoprotein B/gB Protein (HEK293, Fc)

The gB Protein is an envelope glycoprotein that plays a role in host cell entry, cell-to-cell viral transmission, and infected cell fusion. gB Protein interacts with heparin sulfate proteoglycan and forms an association with gH/gL heterodimer. HCMV Glycoprotein B/gB Protein (HEK293, Fc) is the recombinant virus-derived HCMV Glycoprotein B/gB protein, expressed by HEK293, with C-hFc labeled tag and cleavage site mutated RTKR to TTQT.

gB is an envelope glycoprotein that plays a role in host cell entry, cell-to-cell viral transmission, and infected cell fusion. gB is involved in initial attachment through a gM/gN complex bound to heparin sulfate, which binds heparin with higher affinity. gB plays a key role in the fusion of viruses and cell membranes, facilitating the entry of viruses into host cells. The complex fusion mechanisms required for this process include at least gB and gH/gL heterodimers. gB interacts with host integrins ITGB1, PDGFRA, and EGFR, possibly entering the receptor as post-attachment^[1][2][3].

Measured by its ability to bind biotinylated Human CD209-Fc in functional ELISA.

Virus

HEK293

C-hFc

AAA45920 (S25-K700&R777-V907, with cleavage site mutated RTKR to TTQT)

/

160-170 kDa

≥ 80%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Feire AL, et al. The glycoprotein B disintegrin-like domain binds beta 1 integrin to mediate cytomegalovirus entry. J Virol. 2010 Oct;84(19):10026-37.  [Content Brief]

  [2]. Soroceanu L, et al. Platelet-derived growth factor-alpha receptor activation is required for human cytomegalovirus infection. Nature. 2008 Sep 18;455(7211):391-5.  [Content Brief]

  [3]. Wang X, et al. Epidermal growth factor receptor is a cellular receptor for human cytomegalovirus. Nature. 2003 Jul 24;424(6947):456-61.  [Content Brief]