HCoV-229E Nucleoprotein/NP Protein (NP_073556, His)

H-CoV Nucleoprotein (NP, N) is a homodimer with nonspecific binding activity toward nucleic acids. NP packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly, enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication through its interactions with the viral genome and membrane protein M. HCoV-229E Nucleoprotein/NP Protein (NP_073556, His) is the recombinant Virus-derived HCoV-229E Nucleoprotein/NP protein, expressed by E. coli , with N-His labeled tag. The total length of HCoV-229E Nucleoprotein/NP Protein (NP_073556, His) is 389 a.a., with molecular weight of ~44.43 KDa.

H-CoV Nucleoprotein (NP, N) is a homodimer composed of 389 amino acids in each chain. NP can be divided into two structural domains interspersed with disordered (unstructured) regions: the N-terminal domain (NTD; also called RBD) serves as a putative RNA-binding domain, while the C-terminal domain (CTD; also called DD) is a dimerization domain, both the NTD and the CTD bind to nucleic acids through electropositive regions on their surfaces.
NP is one of the most abundant coronavirus proteins with nonspecific binding activity toward nucleic acids, including ssRNA, single-stranded DNA, and double-stranded DNA, NP can also act as an RNA chaperone. NP packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. NP plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication^[1].

Virus

E. coli

N-His

NP_073556 (M1-N389)

918763  [NCBI]

Approximately 44.43 kDa.

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.