HCoV-HKU1 Spike/S1 Protein (Q5MQD0, HEK293, His)

The HCoV-HKU1 Spike/S1 Protein plays a pivotal role in infection by attaching the virion to the host cell membrane, initiating the infection process. As a class I viral fusion protein, it facilitates membrane fusion between the virion and host cell through dynamic conformational transitions. The coiled coil regions, with heptad repeats, form a trimer-of-hairpins structure during fusion, bringing the fusion peptide close to the C-terminal region and driving the fusion of viral and target cell membranes. HCoV-HKU1 Spike/S1 Protein (Q5MQD0, HEK293, His) is the recombinant Virus-derived HCoV-HKU1 Spike/S1 protein, expressed by HEK293, with C-His labeled tag. The total length of HCoV-HKU1 Spike/S1 Protein (Q5MQD0, HEK293, His) is 760 a.a., with molecular weight of 130-140 KDa.

The HCoV-HKU1 Spike/S1 protein plays a pivotal role in the infection process by attaching the virion to the host cell membrane through interaction with the host receptor, initiating the infection. Functioning as a class I viral fusion protein, it facilitates the fusion of virion and cellular membranes. The protein undergoes a dynamic conformational transition, exhibiting at least three states: the pre-fusion native state, the pre-hairpin intermediate state, and the post-fusion hairpin state. During the fusion process, the coiled coil regions, characterized by heptad repeats, form a trimer-of-hairpins structure. This arrangement positions the fusion peptide in close proximity to the C-terminal region of the ectodomain, a configuration believed to drive the apposition and subsequent fusion of viral and target cell membranes.

Virus

HEK293

C-His

Q5MQD0 (M1-R760)

3200426  [NCBI]

130-140 kDa.

Greater than 90% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.