1. Recombinant Proteins
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  3. HCoV-NL63 Spike/S1 Protein (HEK293, His)

HCoV-NL63 Spike/S1 Protein (HEK293, His)

Cat. No.: HY-P76978
Handling Instructions

HCoV-NL63 is a coronavirus, specifically a filovirus from the genus coronavirus A. HCoV-NL63 is a coated, positive single-stranded RNA virus that enters host cells by binding to ACE2. HCoV-NL63 is mainly found in young children, the elderly, and immunocompromised patients with acute respiratory diseases. HCoV-NL63 Spike/S1 Protein (HEK293, His) is the recombinant Virus-derived HCoV-NL63 Spike/S1 protein, expressed by HEK293 , with C-His labeled tag. The total length of HCoV-NL63 Spike/S1 Protein (HEK293, His) is 699 a.a., with molecular weight of ~78.8 KDa.

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Description

HCoV-NL63 is a coronavirus, specifically a filovirus from the genus coronavirus A. HCoV-NL63 is a coated, positive single-stranded RNA virus that enters host cells by binding to ACE2. HCoV-NL63 is mainly found in young children, the elderly, and immunocompromised patients with acute respiratory diseases. HCoV-NL63 Spike/S1 Protein (HEK293, His) is the recombinant Virus-derived HCoV-NL63 Spike/S1 protein, expressed by HEK293 , with C-His labeled tag. The total length of HCoV-NL63 Spike/S1 Protein (HEK293, His) is 699 a.a., with molecular weight of ~78.8 KDa.

Background

HCoV-NL63 is a coronavirus, specifically a filovirus from the genus coronavirus A. The virus is a coated, positive single-stranded RNA virus that enters host cells by binding to ACE2. The S1 region attaches virions to the cell membrane through interactions with host receptors, triggering infection. Binding to the receptor may cause conformational changes of S glycoprotein, reveal fusion peptide in the S2 region, and activate membrane fusion. The S2 region belongs to the Class I virus fusion protein. The virus is mainly found in young children, the elderly and immunocompromised patients with acute respiratory illness[1][2][3].

Species

Virus

Source

HEK293

Tag

C-His

Accession

APF29071 (C19-V717)

Gene ID

/

Molecular Construction
N-term
HCoV-NL63 S1 (C19-V717)
Accession # APF29071
His
C-term
Synonyms
Human coronavirus (HCoV-NL63) Spike/S1 Protein (S1 Subunit, His)
Molecular Weight

Approximately 78.8 kDa.

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

HCoV-NL63 Spike/S1 Protein (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
HCoV-NL63 Spike/S1 Protein (HEK293, His)
Cat. No.:
HY-P76978
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