1. Recombinant Proteins
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Hemagglutinin
  5. HA/Hemagglutinin Protein, H10N3 (BAF46762, sf9, His)

HA/Hemagglutinin Protein, H10N3 (BAF46762, sf9, His)

Cat. No.: HY-P75867
Handling Instructions

Influenza A virus is a single-stranded, negative-sense, segmented RNA virus of the family Orthomyxoviridae. Its genome harbours eight viral RNA (vRNA) segments encoding at least 17 proteins. HA functions as the receptor binding glycoprotein that binds to cell-surface sialylated glycoproteins. H10N3 avian influenza viruses (AIVs) bind to α-2, 3-linked sialic acids with high affinity and to α-2, 6-linked sialic acids with affinity. HA/Hemagglutinin Protein, H10N3 (BAF46762, sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of HA/Hemagglutinin Protein, H10N3 (BAF46762, sf9, His) is 525 a.a., with molecular weight of ~57.9 kDa.

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Description

Influenza A virus is a single-stranded, negative-sense, segmented RNA virus of the family Orthomyxoviridae. Its genome harbours eight viral RNA (vRNA) segments encoding at least 17 proteins. HA functions as the receptor binding glycoprotein that binds to cell-surface sialylated glycoproteins. H10N3 avian influenza viruses (AIVs) bind to α-2, 3-linked sialic acids with high affinity and to α-2, 6-linked sialic acids with affinity[1][2][3]. HA/Hemagglutinin Protein, H10N3 (BAF46762, sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of HA/Hemagglutinin Protein, H10N3 (BAF46762, sf9, His) is 525 a.a., with molecular weight of ~57.9 kDa.

Background

Influenza A viruses are categorized into 18 different HA subtypes and 11 different NA subtypes based on the different antigenicity of their two surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA). Influenza viruses are prone to genetic mutations during replication, and sometimes one or two amino acid mutations can cause huge biological changes[1].
HA functions as the receptor binding glycoprotein that binds to cell-surface sialylated glycoproteins. Particularly, influenza viruses predominantly bind to α2,3-linked sialic acid (SA) receptors abundantly located in the lower respiratory tract of birds and the intestinal tract with respect to avian influenza viruses, to α2,6-linked SA receptors. Upon virus attachment, a conformational change in the HA protein is required for the fusion reaction between the viral and endosomal membranes, after which viral ribonucleoproteins are released in the cytoplasm of the infected cell[2].

Species

Virus

Source

Sf9 insect cells

Tag

C-His

Accession

BAF46762 (M1-I525)

Gene ID

/

Molecular Construction
N-term
H10N3 HA (M1-I525)
Accession # BAF46762
His
C-term
Synonyms
Influenza A H10N3 (A/duck/Hong Kong/786/1979) Hemagglutinin / HA Protein (His)
Molecular Weight

Approximately 57.9 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

HA/Hemagglutinin Protein, H10N3 (BAF46762, sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
HA/Hemagglutinin Protein, H10N3 (BAF46762, sf9, His)
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HY-P75867
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