HA/Hemagglutinin Protein, H14N5 (P26136, sf9, His)

The HA1/Hemagglutinin protein binds cell surface receptors, facilitating virus attachment. It determines host range and virulence, mediating fusion of viral and endosomal membranes. Acidic endosomes trigger HA2 conformational change, releasing fusion peptide. Multiple HA1 trimers form fusion pore. Hemagglutinin/HA Protein, H14N5 (P26136, sf9, His) is the recombinant Virus-derived Hemagglutinin/HA protein, expressed by Sf9 insect cells , with C-His labeled tag.

The HA1/Hemagglutinin protein binds to receptors on the cell surface that contain sialic acid, facilitating the attachment of the virus particle to the cell. This attachment leads to internalization of approximately two thirds of the virus particles through a process called clathrin-dependent endocytosis, while about one third enters through a clathrin- and caveolin-independent pathway. The HA1 protein plays a crucial role in determining host range restriction and virulence of the virus. As a class I viral fusion protein, it enables the virus to penetrate the cell cytoplasm by mediating the fusion of the viral membrane with the endosomal membrane. The acidic environment in endosomes triggers a irreversible conformational change in the HA2 subunit, causing the release of a hydrophobic peptide required for fusion. The formation of a competent fusion pore necessitates the presence of multiple trimers of the HA1/Hemagglutinin protein.

Virus

Sf9 insect cells

C-His

P26136 (Q18-D531)

/

Extracellular Domain

QITNGTTGNPIICLGHHAVENGTSVKTLTDNHVEVVSAKELVETNHTDELCPSPLKLVDGQDCHLINGALGSPGCDRLQDTTWDVFIERPTAVDTCYPFDVPDYQSLRSILASSGSLEFIAEQFTWNGVKVDGSSSACLRGGRNSFFSRLNWLTKATNGNYGPINVTKENTGSYVRLYLWGVHHPSSDNEQTDLYKVATGRVTVSTRSDQISIVPNIGSRPRVRNQSGRISIYWTLVNPGDSIIFNSIGNLIAPRGHYKISKSTKSTVLKSDKRIGSCTSPCLTDKGSIQSDKPFQNVSRIAIGNCPKYVKQGSLMLATGMRNIPGKQAKGLFGAIAGFIENGWQGLIDGWYGFRHQNAEGTGTAADLKSTQAAIDQINGKLNRLIEKTNEKYHQIEKEFEQVEGRIQDLEKYVEDTKIDLWSYNAELLVALENQHTIDVTDSEMNKLFERVRRQLRENAEDQGNGCFEIFHQCDNNCIESIRNGTYDHNIYRDEAINNRIKINPVTLTMGYKD

Approximately 58.6 kDa.

≥ 90%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.